Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DOZ91_RS25080 Genome accession   NZ_CP030063
Coordinates   5229529..5230605 (+) Length   358 a.a.
NCBI ID   WP_127778750.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain ZB201705     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5224529..5235605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOZ91_RS25050 (DOZ91_25480) - 5225301..5225474 (-) 174 WP_034308396.1 DUF2759 domain-containing protein -
  DOZ91_RS25055 (DOZ91_25485) - 5225708..5226334 (+) 627 WP_053535700.1 MBL fold metallo-hydrolase -
  DOZ91_RS25060 (DOZ91_25490) - 5226647..5227747 (+) 1101 WP_164855111.1 SAM-dependent methyltransferase -
  DOZ91_RS25065 (DOZ91_25495) - 5227800..5228042 (-) 243 WP_034308393.1 DUF2626 domain-containing protein -
  DOZ91_RS25070 (DOZ91_25500) - 5228128..5228829 (-) 702 WP_127778472.1 helix-turn-helix domain-containing protein -
  DOZ91_RS25075 (DOZ91_25505) - 5229045..5229425 (+) 381 WP_127778473.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DOZ91_RS25080 (DOZ91_25510) comGA 5229529..5230605 (+) 1077 WP_127778750.1 competence type IV pilus ATPase ComGA Machinery gene
  DOZ91_RS25085 (DOZ91_25515) comGB 5230589..5231623 (+) 1035 WP_164855112.1 competence type IV pilus assembly protein ComGB -
  DOZ91_RS25090 (DOZ91_25520) comGC 5231701..5232012 (+) 312 WP_034308384.1 competence type IV pilus major pilin ComGC -
  DOZ91_RS25095 (DOZ91_25525) comGD 5232020..5232463 (+) 444 WP_164855113.1 competence type IV pilus minor pilin ComGD -
  DOZ91_RS25100 (DOZ91_25530) - 5232450..5232788 (+) 339 WP_127778476.1 hypothetical protein -
  DOZ91_RS25105 (DOZ91_25535) comGF 5232736..5233215 (+) 480 WP_084786101.1 competence type IV pilus minor pilin ComGF -
  DOZ91_RS25110 (DOZ91_25540) comGG 5233208..5233594 (+) 387 WP_053535692.1 competence type IV pilus minor pilin ComGG -
  DOZ91_RS25115 (DOZ91_25545) - 5233710..5233895 (+) 186 WP_048688303.1 YqzE family protein -
  DOZ91_RS25120 (DOZ91_25550) - 5233941..5234738 (-) 798 WP_054400219.1 YqhG family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39858.46 Da        Isoelectric Point: 8.6291

>NTDB_id=298885 DOZ91_RS25080 WP_127778750.1 5229529..5230605(+) (comGA) [Peribacillus frigoritolerans strain ZB201705]
MISIEKTAEKILTRAIVESASDIHIFFRGEGPHIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDYALACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVGYRQLKDEIGKAVAMGYVESQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=298885 DOZ91_RS25080 WP_127778750.1 5229529..5230605(+) (comGA) [Peribacillus frigoritolerans strain ZB201705]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAATCGGCATCGGATATCCACATTTTTTT
TCGCGGAGAGGGGCCTCACATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTGTCATTCTTTGAAGCTG
AAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACACTATATTCCCTGCTTCACCAC
GCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCAGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCGGTCCGTGCCGCATTAACCGGTCATCTGATACTAACGACCATGCAT
ACGAGGGACGCCCAAGGCGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCGCAGCGGCTGGTTGAATTGCGATGTCTTCCGTGTAAAGGGGACTATGCTTTAGCTTGCAAAATGACTGCAA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCGGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCTGTTGCGATGGGCTATGTGGAATCTCAGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

38.218

97.207

0.372
  pilB Haemophilus influenzae Rd KW20

37.069

97.207

0.36

Multiple sequence alignment