Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   DOU31_RS04585 Genome accession   NZ_CP030042
Coordinates   926518..927045 (+) Length   175 a.a.
NCBI ID   WP_010776361.1    Uniprot ID   -
Organism   Enterococcus faecalis strain C25     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 916790..977635 926518..927045 within 0


Gene organization within MGE regions


Location: 916790..977635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU31_RS04500 (DOU31_04495) - 916790..917926 (-) 1137 WP_002405155.1 tyrosine-type recombinase/integrase -
  DOU31_RS04505 (DOU31_04500) - 917993..918565 (-) 573 WP_010776364.1 hypothetical protein -
  DOU31_RS04510 (DOU31_04505) - 918626..918826 (-) 201 WP_002389192.1 hypothetical protein -
  DOU31_RS04515 (DOU31_04510) - 918850..919551 (-) 702 WP_002404917.1 DUF5067 domain-containing protein -
  DOU31_RS04520 (DOU31_04515) - 919640..920155 (-) 516 WP_002404918.1 ImmA/IrrE family metallo-endopeptidase -
  DOU31_RS04525 (DOU31_04520) - 920130..920702 (-) 573 WP_002404919.1 helix-turn-helix domain-containing protein -
  DOU31_RS04530 (DOU31_04525) - 920861..921094 (+) 234 WP_002389146.1 DUF739 family protein -
  DOU31_RS04535 (DOU31_04530) - 921176..921895 (+) 720 WP_002404920.1 phage antirepressor KilAC domain-containing protein -
  DOU31_RS04540 (DOU31_04535) - 921911..922159 (+) 249 WP_002389279.1 hypothetical protein -
  DOU31_RS15160 (DOU31_04540) - 922181..922354 (+) 174 WP_002404922.1 hypothetical protein -
  DOU31_RS15165 - 922462..922617 (+) 156 WP_002395694.1 hypothetical protein -
  DOU31_RS04555 (DOU31_04550) - 922674..922910 (+) 237 WP_002389004.1 hypothetical protein -
  DOU31_RS04560 (DOU31_04555) - 922911..923588 (+) 678 WP_002404924.1 ERF family protein -
  DOU31_RS04565 (DOU31_04560) - 923573..924511 (+) 939 WP_002404925.1 DUF1351 domain-containing protein -
  DOU31_RS04570 (DOU31_04565) - 924508..925188 (+) 681 WP_002404926.1 putative HNHc nuclease -
  DOU31_RS04575 (DOU31_04570) - 925207..926220 (+) 1014 WP_048670087.1 hypothetical protein -
  DOU31_RS04580 (DOU31_04575) - 926220..926528 (+) 309 WP_002381633.1 MazG-like family protein -
  DOU31_RS04585 (DOU31_04580) ssb 926518..927045 (+) 528 WP_010776361.1 single-stranded DNA-binding protein Machinery gene
  DOU31_RS04590 (DOU31_04585) - 927057..927386 (+) 330 WP_078122599.1 hypothetical protein -
  DOU31_RS04595 (DOU31_04590) - 927406..927612 (+) 207 WP_025190349.1 hypothetical protein -
  DOU31_RS04600 (DOU31_04595) - 927666..928148 (+) 483 WP_078122598.1 class I SAM-dependent methyltransferase -
  DOU31_RS04615 (DOU31_04610) - 928558..928905 (+) 348 WP_164978499.1 hypothetical protein -
  DOU31_RS04620 (DOU31_04615) - 928905..929129 (+) 225 WP_078122597.1 hypothetical protein -
  DOU31_RS04625 (DOU31_04620) - 929144..929380 (+) 237 WP_078122596.1 hypothetical protein -
  DOU31_RS04630 (DOU31_04625) - 929386..929709 (+) 324 WP_089201922.1 DNA-directed RNA polymerase subunit delta -
  DOU31_RS04635 (DOU31_04630) - 929706..929957 (+) 252 WP_078122594.1 hypothetical protein -
  DOU31_RS04640 (DOU31_04635) - 929969..930208 (+) 240 WP_074653948.1 hypothetical protein -
  DOU31_RS04645 (DOU31_04640) - 930211..930702 (+) 492 WP_078122593.1 DUF1642 domain-containing protein -
  DOU31_RS15170 - 930703..930864 (+) 162 WP_164978496.1 hypothetical protein -
  DOU31_RS15175 (DOU31_04645) - 930864..931034 (+) 171 WP_010776353.1 hypothetical protein -
  DOU31_RS04660 (DOU31_04655) - 931457..931873 (+) 417 WP_002357362.1 ArpU family phage packaging/lysis transcriptional regulator -
  DOU31_RS15280 (DOU31_04665) - 933454..933678 (+) 225 WP_002404955.1 hypothetical protein -
  DOU31_RS04675 (DOU31_04670) terS 933731..934525 (+) 795 WP_002404957.1 phage terminase small subunit -
  DOU31_RS04680 (DOU31_04675) - 934497..935816 (+) 1320 WP_138807301.1 PBSX family phage terminase large subunit -
  DOU31_RS04685 (DOU31_04680) - 935834..937372 (+) 1539 WP_002404959.1 phage portal protein -
  DOU31_RS04690 (DOU31_04685) - 937377..938315 (+) 939 WP_002404960.1 minor capsid protein -
  DOU31_RS04695 (DOU31_04690) - 938316..938585 (+) 270 WP_002404961.1 hypothetical protein -
  DOU31_RS04700 (DOU31_04695) - 938680..938943 (+) 264 WP_025188184.1 DUF6275 family protein -
  DOU31_RS04705 (DOU31_04700) - 939066..939665 (+) 600 WP_002404963.1 DUF4355 domain-containing protein -
  DOU31_RS04710 (DOU31_04705) - 939678..940610 (+) 933 WP_002404964.1 phage major capsid protein -
  DOU31_RS04715 (DOU31_04710) - 940684..941016 (+) 333 WP_002404965.1 phage head-tail connector protein -
  DOU31_RS04720 (DOU31_04715) - 941013..941348 (+) 336 WP_002404966.1 hypothetical protein -
  DOU31_RS04725 (DOU31_04720) - 941323..941703 (+) 381 WP_002402384.1 HK97-gp10 family putative phage morphogenesis protein -
  DOU31_RS04730 (DOU31_04725) - 941700..942089 (+) 390 WP_002404967.1 hypothetical protein -
  DOU31_RS04735 (DOU31_04730) - 942105..942635 (+) 531 WP_002404968.1 phage major tail protein, TP901-1 family -
  DOU31_RS04740 (DOU31_04735) - 942713..943171 (+) 459 WP_002404969.1 hypothetical protein -
  DOU31_RS04745 (DOU31_04740) - 943227..943577 (+) 351 WP_002404970.1 tail assembly chaperone -
  DOU31_RS04750 (DOU31_04745) - 943670..943927 (+) 258 WP_002404971.1 hypothetical protein -
  DOU31_RS04755 (DOU31_04750) - 943943..947350 (+) 3408 WP_010776350.1 tape measure protein -
  DOU31_RS04760 (DOU31_04755) - 947351..948280 (+) 930 WP_002373078.1 distal tail protein Dit -
  DOU31_RS04765 (DOU31_04760) - 948297..950315 (+) 2019 WP_002404973.1 phage tail protein -
  DOU31_RS15180 - 950316..950489 (+) 174 WP_002404974.1 hypothetical protein -
  DOU31_RS04770 (DOU31_04765) - 950501..952885 (+) 2385 WP_236922054.1 glycerophosphodiester phosphodiesterase family protein -
  DOU31_RS04775 (DOU31_04770) - 952977..953204 (+) 228 WP_002381665.1 hypothetical protein -
  DOU31_RS04780 (DOU31_04775) - 953201..953404 (+) 204 WP_002369080.1 phage holin -
  DOU31_RS04785 (DOU31_04780) - 953409..954668 (+) 1260 WP_002404976.1 LysM peptidoglycan-binding domain-containing protein -
  DOU31_RS04790 (DOU31_04785) - 954770..955366 (-) 597 WP_002404977.1 hypothetical protein -
  DOU31_RS04795 (DOU31_04790) - 955378..955767 (-) 390 WP_002404978.1 DUF6941 family protein -
  DOU31_RS04805 (DOU31_04800) - 956069..957031 (+) 963 WP_002404979.1 Abi family protein -
  DOU31_RS04810 (DOU31_04805) - 957127..957375 (-) 249 WP_002404980.1 hypothetical protein -
  DOU31_RS04820 (DOU31_04815) - 957709..958089 (-) 381 WP_002356881.1 PH domain-containing protein -
  DOU31_RS04825 (DOU31_04820) - 958347..959585 (-) 1239 WP_138807084.1 aminopeptidase -
  DOU31_RS04830 (DOU31_04825) - 959836..960408 (+) 573 WP_002356883.1 TetR/AcrR family transcriptional regulator -
  DOU31_RS04835 (DOU31_04830) - 960566..961039 (+) 474 WP_002356884.1 TspO/MBR family protein -
  DOU31_RS04840 (DOU31_04835) - 961113..961556 (-) 444 WP_002356885.1 flavodoxin -
  DOU31_RS04845 (DOU31_04840) map 961731..962495 (+) 765 WP_002356886.1 type I methionyl aminopeptidase -
  DOU31_RS04850 (DOU31_04845) - 962511..963419 (+) 909 WP_002383768.1 YihY/virulence factor BrkB family protein -
  DOU31_RS04855 (DOU31_04850) - 963530..964666 (+) 1137 WP_002366956.1 MraY family glycosyltransferase -
  DOU31_RS04860 (DOU31_04855) - 964788..965576 (+) 789 WP_002359892.1 glycosyltransferase family 2 protein -
  DOU31_RS04865 (DOU31_04860) - 965576..966403 (+) 828 WP_002369091.1 glycosyltransferase -
  DOU31_RS04870 (DOU31_04865) - 966407..967120 (+) 714 WP_002356891.1 glycosyltransferase family 2 protein -
  DOU31_RS04875 (DOU31_04870) rfbA 967242..968108 (+) 867 WP_002364387.1 glucose-1-phosphate thymidylyltransferase RfbA -
  DOU31_RS04880 (DOU31_04875) rfbC 968121..968693 (+) 573 WP_002356893.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  DOU31_RS04885 (DOU31_04880) rfbB 968718..969746 (+) 1029 WP_002359895.1 dTDP-glucose 4,6-dehydratase -
  DOU31_RS04890 (DOU31_04885) rfbD 969839..970681 (+) 843 WP_002370379.1 dTDP-4-dehydrorhamnose reductase -
  DOU31_RS04895 (DOU31_04890) - 970740..971465 (+) 726 WP_002359897.1 glycosyltransferase family 2 protein -
  DOU31_RS04900 (DOU31_04895) - 971465..971815 (+) 351 WP_002370376.1 DUF2304 domain-containing protein -
  DOU31_RS04905 (DOU31_04900) - 971889..972230 (-) 342 WP_002356899.1 EamA family transporter -
  DOU31_RS04910 (DOU31_04905) - 972478..973272 (+) 795 WP_002356900.1 ABC transporter permease -
  DOU31_RS04915 (DOU31_04910) - 973285..974502 (+) 1218 WP_002356901.1 ABC transporter ATP-binding protein -
  DOU31_RS04920 (DOU31_04915) - 974492..977635 (+) 3144 WP_010717058.1 glycosyltransferase -

Sequence


Protein


Download         Length: 175 a.a.        Molecular weight: 19345.20 Da        Isoelectric Point: 4.7078

>NTDB_id=298679 DOU31_RS04585 WP_010776361.1 926518..927045(+) (ssb) [Enterococcus faecalis strain C25]
MINNVVLIGRLTKDIDLRYTASGSAVGSFTLAVNRNFTNQNGEREADFINCVIWRKPAETMANYARKGTLLGVVGRIQTR
NYDNQQGQRVYVTEVVCESFQLLESKSTNENRNSVQSSQNSVTGVQNNFESNYATNQNKGLNQQNNSQQMSFGGDVDPFA
GAGNSIDISDDDLPF

Nucleotide


Download         Length: 528 bp        

>NTDB_id=298679 DOU31_RS04585 WP_010776361.1 926518..927045(+) (ssb) [Enterococcus faecalis strain C25]
ATGATAAATAATGTGGTATTAATCGGAAGGCTGACGAAAGATATAGATTTACGCTACACCGCAAGTGGTTCTGCAGTTGG
AAGCTTTACTCTTGCTGTGAACCGTAACTTTACAAACCAAAACGGCGAACGAGAAGCGGATTTTATCAACTGTGTAATTT
GGCGTAAGCCTGCTGAAACAATGGCTAATTATGCTCGCAAAGGAACATTATTAGGAGTTGTTGGAAGAATTCAAACTCGT
AATTATGACAACCAACAAGGCCAACGTGTGTATGTGACTGAAGTTGTTTGCGAAAGCTTCCAATTATTAGAGTCAAAAAG
CACCAATGAGAATAGAAATAGCGTTCAGAGTTCGCAGAATAGCGTTACAGGCGTTCAAAATAATTTCGAGAGTAATTATG
CCACGAATCAAAACAAAGGCTTAAATCAGCAAAATAACAGCCAACAAATGTCGTTTGGTGGAGATGTAGATCCGTTCGCA
GGTGCAGGTAATTCAATCGACATTAGCGATGATGATCTGCCTTTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.551

100

0.606

  ssbA Bacillus subtilis subsp. subtilis str. 168

57.865

100

0.589


Multiple sequence alignment