Detailed information NTG0029638

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: SUB_RS00755
• Genome accession: NC_012004
• Coordinates: 125725..126180 (+)
• Length: 151 a.a.
• NCBI ID: WP_012657678.1
• Uniprot ID: B9DT03
• Organism: Streptococcus uberis 0140J
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 120725..131180

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SUB_RS00725 (SUB0117)	-	122433..122798 (+)	366	WP_041817508.1	DUF1033 family protein	-
SUB_RS00730 (SUB0118)	comGA/cglA	122871..123812 (+)	942	WP_012657673.1	competence type IV pilus ATPase ComGA	Machinery gene
SUB_RS00735 (SUB0119)	comGB	123724..124779 (+)	1056	WP_172631729.1	competence type IV pilus assembly protein ComGB	Machinery gene
SUB_RS00740 (SUB0120)	comGC/cglC	124780..125103 (+)	324	WP_012657675.1	competence type IV pilus major pilin ComGC	Machinery gene
SUB_RS00745 (SUB0121)	comGD	125057..125494 (+)	438	WP_012657676.1	competence type IV pilus minor pilin ComGD	Machinery gene
SUB_RS00750 (SUB0122)	comGE	125466..125765 (+)	300	WP_012657677.1	competence type IV pilus minor pilin ComGE	Machinery gene
SUB_RS00755 (SUB0123)	comGF	125725..126180 (+)	456	WP_012657678.1	competence type IV pilus minor pilin ComGF	Machinery gene
SUB_RS00760 (SUB0124)	comGG	126158..126517 (+)	360	WP_012657679.1	competence type IV pilus minor pilin ComGG	-
SUB_RS00765 (SUB0125)	comGH	126562..127518 (+)	957	WP_012657680.1	class I SAM-dependent methyltransferase	Machinery gene
SUB_RS00770 (SUB0126)	-	127574..128764 (+)	1191	WP_012657681.1	acetate kinase	-
SUB_RS00775 (SUB0127)	-	128882..129085 (+)	204	WP_012657682.1	helix-turn-helix transcriptional regulator	-
SUB_RS00780 (SUB0128)	-	129087..129539 (+)	453	WP_012657683.1	hypothetical protein	-
SUB_RS00785 (SUB0129)	-	129548..130201 (+)	654	WP_012657684.1	CPBP family intramembrane glutamic endopeptidase	-
SUB_RS00790 (SUB0130)	proC	130227..130997 (-)	771	WP_012657685.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 151 a.a.
• Molecular weight: 17647.48 Da
• Isoelectric Point: 10.0997

>NTDB_id=29638 SUB_RS00755 WP_012657678.1 125725..126180(+) (comGF) [Streptococcus uberis 0140J]
MIKKYSKLVACPTKAFTLLEALVALLVISGSLLLYQGLTKSIFSQMTYLRENNQDRWLLFSHQLRAELMGSRFQKVEKNK
IYVTKDGKDVAYGLSKRDDFRKTSANGHGYHPMLLHLSQATIEEKERTVTIHFVWKDGLERTWLYDFKENR

Nucleotide

• Length: 456 bp

>NTDB_id=29638 SUB_RS00755 WP_012657678.1 125725..126180(+) (comGF) [Streptococcus uberis 0140J]
ATGATCAAGAAATACTCAAAATTAGTCGCTTGTCCAACTAAAGCCTTTACACTTTTGGAAGCTCTAGTAGCCTTACTGGT
AATATCAGGGTCTTTGTTACTCTATCAGGGCTTGACCAAATCTATTTTTTCTCAGATGACTTATTTAAGAGAAAACAATC
AGGATAGATGGTTACTCTTTTCTCATCAGTTGCGAGCGGAGTTAATGGGATCTCGTTTTCAGAAAGTTGAAAAGAATAAA
ATTTATGTGACCAAGGATGGTAAGGATGTAGCATATGGGCTTTCAAAAAGGGACGACTTTCGCAAAACATCGGCAAATGG
TCATGGCTATCATCCCATGTTATTACATTTAAGTCAGGCCACAATTGAAGAAAAAGAAAGAACTGTCACCATTCATTTTG
TTTGGAAGGATGGTCTGGAGAGGACATGGCTATATGATTTTAAAGAAAACCGTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-140)

3D structure

• AlphaFold DB: B9DT03

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA140	50	90.066	0.45
comGF/cglF	Streptococcus mitis NCTC 12261	51.515	87.417	0.45
comGF	Streptococcus mutans UA159	49.265	90.066	0.444
comGF/cglF	Streptococcus pneumoniae D39	50.376	88.079	0.444
comGF/cglF	Streptococcus pneumoniae TIGR4	50.376	88.079	0.444
comGF/cglF	Streptococcus pneumoniae R6	50.376	88.079	0.444
comGF/cglF	Streptococcus mitis SK321	50.376	88.079	0.444
comGF/cglF	Streptococcus pneumoniae Rx1	50.376	88.079	0.444
comGF	Lactococcus lactis subsp. cremoris KW2	43.478	91.391	0.397