Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CSC56_RS00715 Genome accession   NZ_CP029652
Coordinates   82157..82654 (+) Length   165 a.a.
NCBI ID   WP_029694423.1    Uniprot ID   -
Organism   Staphylococcus aureus strain AR_0471     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 77157..87654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC56_RS00675 (CSC56_0087) - 77201..78187 (+) 987 WP_000161307.1 ROK family glucokinase -
  CSC56_RS00680 (CSC56_0088) - 78187..78516 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CSC56_RS00685 (CSC56_0089) - 78513..79136 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  CSC56_RS00690 (CSC56_0090) comGA 79188..80162 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  CSC56_RS00695 (CSC56_0091) comGB 80134..81204 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  CSC56_RS00700 (CSC56_0092) comGC 81218..81529 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  CSC56_RS00705 (CSC56_0093) comGD 81507..81953 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  CSC56_RS00710 (CSC56_0094) comGE 81940..82221 (+) 282 WP_000844412.1 hypothetical protein Machinery gene
  CSC56_RS00715 (CSC56_0095) comGF 82157..82654 (+) 498 WP_029694423.1 competence type IV pilus minor pilin ComGF Machinery gene
  CSC56_RS00720 - 82751..82897 (+) 147 WP_001789879.1 hypothetical protein -
  CSC56_RS00725 (CSC56_0096) - 82887..83411 (+) 525 WP_001015120.1 shikimate kinase -
  CSC56_RS00730 (CSC56_0097) gcvT 83570..84661 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  CSC56_RS00735 (CSC56_0098) gcvPA 84681..86027 (+) 1347 WP_103151974.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CSC56_RS00740 (CSC56_0099) gcvPB 86020..87492 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19155.82 Da        Isoelectric Point: 10.0355

>NTDB_id=294533 CSC56_RS00715 WP_029694423.1 82157..82654(+) (comGF) [Staphylococcus aureus strain AR_0471]
MILSKVTNKYVPFQKIPLLINRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=294533 CSC56_RS00715 WP_029694423.1 82157..82654(+) (comGF) [Staphylococcus aureus strain AR_0471]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAATAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

95.152

100

0.952

Multiple sequence alignment