Detailed information NTG0294141

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DL538_RS13255
• Genome accession: NZ_CP029609
• Coordinates: 2568698..2569315 (-)
• Length: 205 a.a.
• NCBI ID: WP_131227639.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain G7
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2563698..2574315

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DL538_RS13225 (DL538_13180)	rpsT	2564000..2564266 (+)	267	WP_131227637.1	30S ribosomal protein S20	-
DL538_RS13230 (DL538_13185)	holA	2564281..2565324 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
DL538_RS13235	-	2565364..2565513 (-)	150	WP_003229985.1	hypothetical protein	-
DL538_RS13240 (DL538_13190)	yqzM	2565554..2565688 (+)	135	WP_003229983.1	YqzM family protein	-
DL538_RS13245 (DL538_13195)	comEC	2565728..2568058 (-)	2331	WP_042976534.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DL538_RS13250 (DL538_13200)	comEB	2568062..2568631 (-)	570	WP_003229978.1	ComE operon protein 2	-
DL538_RS13255 (DL538_13205)	comEA	2568698..2569315 (-)	618	WP_131227639.1	competence protein ComEA	Machinery gene
DL538_RS13260 (DL538_13210)	comER	2569399..2570220 (+)	822	WP_014477389.1	late competence protein ComER	-
DL538_RS13265 (DL538_13215)	yqeM	2570286..2571029 (-)	744	WP_015384134.1	class I SAM-dependent methyltransferase	-
DL538_RS13270 (DL538_13220)	rsfS	2571026..2571382 (-)	357	WP_131227641.1	ribosome silencing factor	-
DL538_RS13275 (DL538_13225)	yqeK	2571400..2571960 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DL538_RS13280 (DL538_13230)	nadD	2571950..2572519 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
DL538_RS13285 (DL538_13235)	yhbY	2572531..2572821 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
DL538_RS13290 (DL538_13240)	aroE	2572815..2573657 (-)	843	WP_131227643.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21795.53 Da
• Isoelectric Point: 4.7220

>NTDB_id=294141 DL538_RS13255 WP_131227639.1 2568698..2569315(-) (comEA) [Bacillus subtilis subsp. subtilis strain G7]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=294141 DL538_RS13255 WP_131227639.1 2568698..2569315(-) (comEA) [Bacillus subtilis subsp. subtilis strain G7]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGTGGAGGGTCTG
TCCAAAGTGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	35.909	100	0.385