Detailed information NTG0292824

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DKG78_RS12755
• Genome accession: NZ_CP029466
• Coordinates: 2616169..2616783 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain HM618
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2611169..2621783

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DKG78_RS12725 (DKG78_12920)	rpsT	2611472..2611738 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DKG78_RS12730 (DKG78_12925)	holA	2611755..2612796 (-)	1042	Protein_2482	DNA polymerase III subunit delta	-
DKG78_RS12735	-	2612836..2612987 (-)	152	Protein_2483	hypothetical protein	-
DKG78_RS12740 (DKG78_12930)	-	2613028..2613162 (+)	135	WP_003152870.1	YqzM family protein	-
DKG78_RS12745 (DKG78_12935)	comEC	2613181..2615532 (-)	2352	WP_015240244.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DKG78_RS12750 (DKG78_12940)	-	2615533..2616102 (-)	570	WP_003152868.1	ComE operon protein 2	-
DKG78_RS12755 (DKG78_12945)	comEA	2616169..2616783 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
DKG78_RS12760 (DKG78_12950)	comER	2616842..2617663 (+)	822	WP_015240246.1	late competence protein ComER	-
DKG78_RS12765 (DKG78_12955)	-	2617732..2618469 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
DKG78_RS12770 (DKG78_12960)	rsfS	2618466..2618822 (-)	357	WP_007408260.1	ribosome silencing factor	-
DKG78_RS12775 (DKG78_12965)	yqeK	2618840..2619400 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DKG78_RS12780 (DKG78_12970)	-	2619390..2619959 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
DKG78_RS12785 (DKG78_12975)	yhbY	2619970..2620260 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DKG78_RS12790 (DKG78_12980)	aroE	2620254..2621096 (-)	843	WP_059368084.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=292824 DKG78_RS12755 WP_015240245.1 2616169..2616783(-) (comEA) [Bacillus amyloliquefaciens strain HM618]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=292824 DKG78_RS12755 WP_015240245.1 2616169..2616783(-) (comEA) [Bacillus amyloliquefaciens strain HM618]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363