Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   DJ572_RS12665 Genome accession   NZ_CP029461
Coordinates   2441345..2441962 (-) Length   205 a.a.
NCBI ID   WP_119899573.1    Uniprot ID   -
Organism   Bacillus subtilis strain QB61     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2436345..2446962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DJ572_RS12635 (DJ572_12595) rpsT 2436647..2436913 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  DJ572_RS12640 (DJ572_12600) holA 2436928..2437971 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  DJ572_RS12645 - 2438011..2438160 (-) 150 WP_003229985.1 hypothetical protein -
  DJ572_RS12650 (DJ572_12605) yqzM 2438201..2438335 (+) 135 WP_003229983.1 YqzM family protein -
  DJ572_RS12655 (DJ572_12610) comEC 2438375..2440705 (-) 2331 WP_119899571.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  DJ572_RS12660 (DJ572_12615) comEB 2440709..2441278 (-) 570 WP_003229978.1 ComE operon protein 2 -
  DJ572_RS12665 (DJ572_12620) comEA 2441345..2441962 (-) 618 WP_119899573.1 competence protein ComEA Machinery gene
  DJ572_RS12670 (DJ572_12625) comER 2442046..2442867 (+) 822 WP_004398597.1 late competence protein ComER -
  DJ572_RS12675 (DJ572_12630) yqeM 2442933..2443676 (-) 744 WP_015714290.1 class I SAM-dependent methyltransferase -
  DJ572_RS12680 (DJ572_12635) rsfS 2443673..2444029 (-) 357 WP_041333759.1 ribosome silencing factor -
  DJ572_RS12685 (DJ572_12640) yqeK 2444047..2444607 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  DJ572_RS12690 (DJ572_12645) nadD 2444597..2445166 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  DJ572_RS12695 (DJ572_12650) yhbY 2445178..2445468 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  DJ572_RS12700 (DJ572_12655) aroE 2445462..2446304 (-) 843 WP_029317883.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21726.42 Da        Isoelectric Point: 4.6246

>NTDB_id=292583 DJ572_RS12665 WP_119899573.1 2441345..2441962(-) (comEA) [Bacillus subtilis strain QB61]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMLT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=292583 DJ572_RS12665 WP_119899573.1 2441345..2441962(-) (comEA) [Bacillus subtilis strain QB61]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCTAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

35.909

100

0.385

Multiple sequence alignment