Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DG474_RS09415 Genome accession   NZ_CP029257
Coordinates   1969514..1970812 (-) Length   432 a.a.
NCBI ID   WP_255778202.1    Uniprot ID   -
Organism   Streptococcus oralis strain CCUG 53468     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1964514..1975812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DG474_RS09395 (DG474_09545) - 1964848..1966821 (-) 1974 WP_255778200.1 DHH family phosphoesterase -
  DG474_RS09400 (DG474_09550) - 1966967..1968145 (-) 1179 WP_255778971.1 acetyl-CoA C-acetyltransferase -
  DG474_RS09405 (DG474_09555) raiA 1968227..1968775 (-) 549 WP_000599111.1 ribosome-associated translation inhibitor RaiA -
  DG474_RS09410 (DG474_09560) comFC/cflB 1968855..1969517 (-) 663 WP_255778201.1 ComF family protein Machinery gene
  DG474_RS09415 (DG474_09565) comFA/cflA 1969514..1970812 (-) 1299 WP_255778202.1 DEAD/DEAH box helicase Machinery gene
  DG474_RS09420 (DG474_09570) - 1970869..1971504 (+) 636 WP_255778203.1 YigZ family protein -
  DG474_RS09425 (DG474_09575) - 1971520..1971963 (+) 444 WP_247940441.1 PH domain-containing protein -
  DG474_RS09430 (DG474_09580) cysK 1972060..1972986 (+) 927 WP_255778204.1 cysteine synthase A -
  DG474_RS09435 (DG474_09585) tsf 1973090..1974130 (-) 1041 WP_255778205.1 translation elongation factor Ts -
  DG474_RS09440 (DG474_09590) rpsB 1974209..1974988 (-) 780 WP_255778206.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49452.25 Da        Isoelectric Point: 7.1272

>NTDB_id=291449 DG474_RS09415 WP_255778202.1 1969514..1970812(-) (comFA/cflA) [Streptococcus oralis strain CCUG 53468]
MKVNPNYLGCLFTEKELTEEERQMAEKLPAMRKEKGKLFCQRCNSSILEEWHLPIGAYYCRECLLMERVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVIDEGGAVCLASPRIDVCLELYKR
LQNDFACEIALLHGESEPYFRTPLVVATTHQLLKFYHAFDLLIVDEVDAFPYVDNSVLYYAVNQCVKEEGLRIFLTATST
DELDKKVRTGELKRLSLPRRFHGNPLIIPKPVWLSDFNRCLEKNQLSTKLKTYIEKQRRTGYPLLIFTSEIKKGEGLKEI
LKVHFPNESIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGELLFFHDGLNASIKKAIKEIKQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=291449 DG474_RS09415 WP_255778202.1 1969514..1970812(-) (comFA/cflA) [Streptococcus oralis strain CCUG 53468]
ATGAAAGTAAATCCAAACTATCTCGGTTGCTTGTTTACTGAGAAAGAATTAACGGAAGAAGAACGGCAGATGGCTGAGAA
ACTTCCAGCAATGAGAAAAGAGAAAGGGAAACTGTTTTGTCAACGTTGTAATAGTAGTATTCTAGAAGAATGGCATTTGC
CTATAGGCGCTTACTATTGTAGGGAGTGTTTATTGATGGAGAGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCACAG
GAGGATTTTCCTAAGCAAGACGTCCTCAAATGGCGTGGTCAGTTAACACCTTTTCAAGAAAAAGTTTCAGAGGGACTTCT
TCAAGCGGTAGACAAGCAAGAGCCAACCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAGATGATTTATCAAGTTG
TGGCTAAAGTGATTGATGAAGGTGGTGCAGTTTGTTTAGCCAGCCCTCGAATAGATGTTTGCTTGGAGTTATATAAGCGA
CTGCAGAATGACTTTGCTTGTGAGATAGCACTACTTCATGGTGAGTCAGAACCCTATTTTCGAACACCACTAGTTGTTGC
AACGACTCATCAGCTGTTAAAATTTTATCATGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAACTCTGTTCTTTACTATGCTGTAAACCAATGTGTAAAGGAGGAGGGGTTAAGGATATTTCTTACAGCAACTTCTACA
GATGAGTTAGATAAGAAGGTTCGCACTGGAGAATTAAAACGATTGAGCTTGCCAAGACGATTTCATGGAAATCCATTGAT
TATTCCTAAACCAGTCTGGTTATCGGATTTTAATCGCTGTTTAGAGAAAAATCAATTGTCAACCAAGTTAAAGACTTATA
TTGAGAAACAGAGAAGAACAGGTTATCCCTTACTGATTTTTACATCAGAGATTAAGAAAGGTGAGGGACTAAAAGAAATA
CTAAAGGTGCATTTCCCGAATGAGAGCATTGGTTTTGTATCTTCTGTGACAGAAGACCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTAACAATACTGATCAGTACAACAATATTGGAACGCGGAGTTACCTTCCCTTGTGTAGATGTTTTCG
TAGTAGAAGCTAATCATCGTCTCTTTACCAAGTCTAGCTTGATTCAGATTGGTGGTCGAGTTGGGCGTAGTATGGACAGA
CCGACTGGTGAGTTGCTCTTCTTTCATGATGGATTAAATGCTTCCATCAAGAAGGCAATAAAGGAAATCAAGCAGATGAA
CAAGGAGGCTGGATTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-168)

Helicase conserved C-terminal domain (Helicase_C)

(292-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

90.046

100

0.9
  comFA/cflA Streptococcus pneumoniae Rx1

89.815

100

0.898
  comFA/cflA Streptococcus pneumoniae D39

89.815

100

0.898
  comFA/cflA Streptococcus pneumoniae R6

89.815

100

0.898
  comFA/cflA Streptococcus pneumoniae TIGR4

89.583

100

0.896
  comFA/cflA Streptococcus mitis SK321

89.352

100

0.894
  comFA Lactococcus lactis subsp. cremoris KW2

51.225

94.444

0.484
  comFA Latilactobacillus sakei subsp. sakei 23K

40.144

96.296

0.387

Multiple sequence alignment