Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CSC48_RS00725 Genome accession   NZ_CP029086
Coordinates   139818..140315 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain AR462     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 134818..145315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC48_RS00700 (CSC48_0102) gcvPB 134980..136452 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  CSC48_RS00705 (CSC48_0103) gcvPA 136445..137791 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CSC48_RS00710 (CSC48_0104) gcvT 137811..138902 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  CSC48_RS00715 (CSC48_0105) - 139061..139585 (-) 525 WP_001015120.1 shikimate kinase -
  CSC48_RS00720 - 139575..139721 (-) 147 WP_001792109.1 hypothetical protein -
  CSC48_RS00725 (CSC48_0106) comGF 139818..140315 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  CSC48_RS00730 (CSC48_0107) comGE 140233..140532 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  CSC48_RS00735 (CSC48_0108) comGD 140519..140965 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  CSC48_RS00740 (CSC48_0109) comGC 140943..141254 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  CSC48_RS00745 (CSC48_0110) comGB 141268..142338 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  CSC48_RS00750 (CSC48_0111) comGA 142310..143284 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  CSC48_RS00755 (CSC48_0112) - 143336..143959 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  CSC48_RS00760 (CSC48_0113) - 143956..144285 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CSC48_RS00765 (CSC48_0114) - 144285..145271 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=290242 CSC48_RS00725 WP_001788897.1 139818..140315(-) (comGF) [Staphylococcus aureus strain AR462]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=290242 CSC48_RS00725 WP_001788897.1 139818..140315(-) (comGF) [Staphylococcus aureus strain AR462]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment