Detailed information NTG0288991

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DBK22_RS08000
• Genome accession: NZ_CP028961
• Coordinates: 1653162..1653776 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM102752
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1648162..1658776

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DBK22_RS07970 (DBK22_01608)	rpsT	1648465..1648731 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DBK22_RS07975 (DBK22_01609)	holA	1648748..1649789 (-)	1042	Protein_1589	DNA polymerase III subunit delta	-
DBK22_RS07980	-	1649829..1649980 (-)	152	Protein_1590	hypothetical protein	-
DBK22_RS07985	-	1650021..1650155 (+)	135	WP_003152870.1	YqzM family protein	-
DBK22_RS07990 (DBK22_01610)	comEC	1650174..1652525 (-)	2352	WP_160242161.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DBK22_RS07995 (DBK22_01611)	-	1652526..1653095 (-)	570	WP_003152868.1	ComE operon protein 2	-
DBK22_RS08000 (DBK22_01612)	comEA	1653162..1653776 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
DBK22_RS08005 (DBK22_01613)	comER	1653835..1654656 (+)	822	WP_015240246.1	late competence protein ComER	-
DBK22_RS08010 (DBK22_01614)	-	1654725..1655462 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
DBK22_RS08015 (DBK22_01615)	rsfS	1655459..1655815 (-)	357	WP_007408260.1	ribosome silencing factor	-
DBK22_RS08020 (DBK22_01616)	yqeK	1655833..1656393 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DBK22_RS08025 (DBK22_01617)	-	1656383..1656952 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
DBK22_RS08030 (DBK22_01618)	yhbY	1656963..1657253 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DBK22_RS08035 (DBK22_01619)	aroE	1657247..1658089 (-)	843	WP_043021640.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=288991 DBK22_RS08000 WP_015240245.1 1653162..1653776(-) (comEA) [Bacillus velezensis strain SRCM102752]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=288991 DBK22_RS08000 WP_015240245.1 1653162..1653776(-) (comEA) [Bacillus velezensis strain SRCM102752]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363