Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   DBO93_RS16095 Genome accession   NZ_CP028924
Coordinates   3740765..3741637 (+) Length   290 a.a.
NCBI ID   WP_108457893.1    Uniprot ID   -
Organism   Colwellia sp. Arc7-D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3735765..3746637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBO93_RS16080 (DBO93_16080) - 3736872..3737318 (+) 447 WP_108457249.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DBO93_RS16085 (DBO93_16085) pilB 3737451..3739156 (+) 1706 Protein_3137 type IV-A pilus assembly ATPase PilB -
  DBO93_RS16090 (DBO93_16090) pilC 3739379..3740632 (+) 1254 WP_108457250.1 type II secretion system F family protein Machinery gene
  DBO93_RS16095 (DBO93_16095) pilD 3740765..3741637 (+) 873 WP_108457893.1 A24 family peptidase Machinery gene
  DBO93_RS16100 (DBO93_16100) coaE 3741744..3742358 (+) 615 WP_108457251.1 dephospho-CoA kinase -
  DBO93_RS16105 (DBO93_16105) zapD 3742477..3743223 (+) 747 WP_108457252.1 cell division protein ZapD -
  DBO93_RS16110 (DBO93_16110) yacG 3743379..3743615 (+) 237 WP_108457253.1 DNA gyrase inhibitor YacG -
  DBO93_RS16115 (DBO93_16115) mutT 3743692..3744090 (-) 399 WP_204100692.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32309.69 Da        Isoelectric Point: 7.9600

>NTDB_id=288594 DBO93_RS16095 WP_108457893.1 3740765..3741637(+) (pilD) [Colwellia sp. Arc7-D]
MITLMTNSPAYFYTIVVVFSLLIGSFLNVVIYRLPKMLEQGWKSECREFLADELAKPIQVDESPITLSTPSSSCPKCQHK
IRFYENIPVISWVFLKGKCSQCKVKISLRYPMVELATAMLSVVIAANYGVTFTTFMLLVLTWGLICLTLIDVDHMLLPDQ
ITLPLLWLGLLVNINGAIVPLHDAVVGAVAGYMSLFSIFWLFKLITGKDGMGHGDFKLVALFGAWIGWQLLPLLILMASA
VGAVIGISLMVFKNHQREQAIPFGPYLAVAGWITLLWGNGIWTWYLSTLT

Nucleotide


Download         Length: 873 bp        

>NTDB_id=288594 DBO93_RS16095 WP_108457893.1 3740765..3741637(+) (pilD) [Colwellia sp. Arc7-D]
ATGATTACCCTGATGACAAATTCTCCAGCTTATTTCTACACTATAGTGGTAGTTTTTTCTTTATTGATTGGGAGCTTTTT
GAATGTTGTTATATATCGCTTACCTAAAATGCTTGAGCAAGGCTGGAAAAGTGAGTGCCGCGAGTTTCTTGCTGATGAAT
TAGCTAAACCCATACAAGTTGATGAAAGCCCAATCACGCTTTCGACACCAAGTTCTAGCTGCCCTAAATGCCAACATAAA
ATTCGTTTTTATGAAAATATTCCCGTTATAAGCTGGGTGTTTTTAAAGGGTAAGTGCAGCCAGTGTAAGGTTAAGATTTC
GCTTCGCTATCCTATGGTTGAGCTTGCCACGGCAATGCTGAGTGTTGTTATTGCAGCTAATTATGGTGTTACTTTCACTA
CATTCATGCTTCTCGTATTAACTTGGGGGCTAATTTGTTTAACCTTGATTGATGTTGATCATATGTTATTGCCTGATCAA
ATTACGCTTCCGCTATTATGGTTAGGTTTATTGGTTAATATAAATGGAGCAATTGTTCCTTTGCATGACGCCGTTGTCGG
TGCTGTTGCCGGTTACATGAGTTTATTCAGCATATTCTGGTTATTTAAGCTAATTACCGGCAAAGACGGTATGGGCCATG
GTGATTTCAAGTTAGTTGCACTGTTTGGGGCATGGATAGGCTGGCAGTTATTACCATTACTCATTCTAATGGCATCGGCC
GTTGGTGCGGTTATCGGCATTAGTTTAATGGTATTTAAAAATCATCAAAGAGAGCAAGCCATTCCTTTTGGTCCATATTT
AGCAGTAGCGGGTTGGATTACGCTGCTTTGGGGTAATGGTATTTGGACTTGGTACTTAAGTACATTAACTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

49.31

100

0.493
  pilD Acinetobacter nosocomialis M2

48.621

100

0.486
  pilD Vibrio cholerae strain A1552

50.178

96.897

0.486
  pilD Vibrio campbellii strain DS40M4

48.936

97.241

0.476
  pilD Neisseria gonorrhoeae MS11

47.292

95.517

0.452

Multiple sequence alignment