Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DBX32_RS24520 Genome accession   NZ_CP028848
Coordinates   5147636..5149336 (+) Length   566 a.a.
NCBI ID   WP_023089630.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain IMP67     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5142636..5154336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBX32_RS24500 (DBX32_24430) - 5143352..5145607 (-) 2256 WP_034020466.1 DUF1631 domain-containing protein -
  DBX32_RS24505 (DBX32_24435) nadC 5145893..5146741 (+) 849 WP_023089629.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DBX32_RS24515 (DBX32_24445) pilA 5146955..5147404 (-) 450 WP_031627735.1 type 4a pilus biogenesis protein PilA Machinery gene
  DBX32_RS24520 (DBX32_24450) pilB 5147636..5149336 (+) 1701 WP_023089630.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DBX32_RS24525 (DBX32_24455) pilC 5149340..5150560 (+) 1221 WP_023089631.1 type 4a pilus biogenesis protein PilC Machinery gene
  DBX32_RS24530 (DBX32_24460) pilD 5150564..5151436 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  DBX32_RS24535 (DBX32_24465) coaE 5151433..5152044 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  DBX32_RS24540 (DBX32_24470) yacG 5152041..5152241 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  DBX32_RS24545 (DBX32_24475) - 5152278..5152487 (-) 210 WP_003094660.1 hypothetical protein -
  DBX32_RS24550 (DBX32_24480) - 5152593..5153282 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  DBX32_RS24555 (DBX32_24485) - 5153279..5153749 (-) 471 WP_003103866.1 hypothetical protein -
  DBX32_RS24560 (DBX32_24490) - 5153746..5154171 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62397.30 Da        Isoelectric Point: 5.7041

>NTDB_id=288096 DBX32_RS24520 WP_023089630.1 5147636..5149336(+) (pilB) [Pseudomonas aeruginosa strain IMP67]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALVELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=288096 DBX32_RS24520 WP_023089630.1 5147636..5149336(+) (pilB) [Pseudomonas aeruginosa strain IMP67]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCTCTGCA
GGCGCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGTCGAATTGTCCGCCGAGCAGTTCGGTATCGCCTATTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCTATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATTCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCTTAGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGATGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTA
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGACGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.58
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

99.293

0.5
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

40.072

97.88

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

93.11

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366

Multiple sequence alignment