Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdL   Type   Machinery gene
Locus tag   MBOO_RS09920 Genome accession   NC_009712
Coordinates   1968368..1970617 (-) Length   749 a.a.
NCBI ID   WP_012107474.1    Uniprot ID   A7I9L0
Organism   Methanoregula boonei 6A8     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1963368..1975617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MBOO_RS09895 (Mboo_1900) - 1964099..1964365 (+) 267 WP_012107468.1 hypothetical protein -
  MBOO_RS09900 (Mboo_1901) - 1964380..1965822 (-) 1443 WP_012107469.1 cobyric acid synthase -
  MBOO_RS09905 (Mboo_1902) yciH 1965941..1966246 (+) 306 WP_012107470.1 stress response translation initiation inhibitor YciH -
  MBOO_RS09910 (Mboo_1903) epdK 1966400..1967449 (-) 1050 WP_012107471.1 type II secretion system F family protein Machinery gene
  MBOO_RS09915 (Mboo_1904) epdJ 1967452..1968126 (-) 675 WP_012107472.1 type II secretion system F family protein Machinery gene
  MBOO_RS14170 (Mboo_1905) epdJ 1968178..1968375 (-) 198 WP_012107473.1 hypothetical protein Machinery gene
  MBOO_RS09920 (Mboo_1906) epdL 1968368..1970617 (-) 2250 WP_012107474.1 type II/IV secretion system ATPase subunit Machinery gene
  MBOO_RS13180 (Mboo_1907) - 1970948..1971685 (-) 738 WP_012107475.1 roadblock/LC7 domain-containing protein -
  MBOO_RS09930 (Mboo_1908) - 1971711..1972058 (-) 348 WP_012107476.1 roadblock/LC7 domain-containing protein -
  MBOO_RS13185 (Mboo_1909) - 1972324..1973055 (+) 732 WP_012107477.1 hypothetical protein -
  MBOO_RS09940 (Mboo_1910) - 1973082..1973645 (+) 564 WP_012107478.1 hypothetical protein -
  MBOO_RS09945 (Mboo_1911) minD 1973726..1974526 (+) 801 WP_012107479.1 cell division ATPase MinD -
  MBOO_RS13190 (Mboo_1912) - 1974577..1975368 (-) 792 WP_012107480.1 response regulator -

Sequence

Protein


Download         Length: 749 a.a.        Molecular weight: 84885.26 Da        Isoelectric Point: 4.7307

>NTDB_id=28789 MBOO_RS09920 WP_012107474.1 1968368..1970617(-) (epdL) [Methanoregula boonei 6A8]
MNAGTPPQEVVPESLPTPDEPVQFPKEATGKPEELDTFDELLEDVPPAQEGVEPPVLSGDDVRAALGKIMGQPEMVPPSA
HSPEPLTASPESELPPEQVVEITEEEDPRENRTSRFFKKKKAEKVGAPTDQIGKSAVTESDERIISADQISEFSGLILPK
GATFHVDEINLHGRMQAFESTGTGSLPPELAEIWKREFSTSGFKDLESELTLADKGTDKGTEKKSWFSALFSAIRSENVE
YDPKIHGPLVDLTFEPKPGIEVMDTYPVNDPYAYVRVIYDHSTHEYTYQVLEPVLSEPEKDLLKELKERLFEILDINTKD
ISKEEARSQLRRSVDEILADYGIKLNPVEREKILYNMHKDFLGDGMIDAIMHDKYIEDISCDGVNTPLFAFHANYESMKT
TLMYHNAEELDSFVTKLAQRAGKYISIAEPILDATMQDGSRIQMTLGQEVTAHGSTFTIRKFKDEPITPTDLIEWHTFAP
LSIAYIWLCVENGKSAIFAGGTASGKTTALNAISLFIPPMAKIVSLEDTREVKLPHPNWIPSVTRDSFDTAGRGEINMYE
LLRAAMRQRPEYIIVGEVRGKECQTLFQAMSTGHVTYSTTHADSVASVVHRIENPPMDVPRNMLSALDFICVQVQARMGG
KRIRRNKQIVEILDIDPRTNELITNEVFRWRSATDEITYSGKSYILEDIMEARGWNENRMREELKRRQEVLEWMRIKKIR
HYKDVAKILISYFREPEVVVERVRKDLYE

Nucleotide


Download         Length: 2250 bp        

>NTDB_id=28789 MBOO_RS09920 WP_012107474.1 1968368..1970617(-) (epdL) [Methanoregula boonei 6A8]
ATGAATGCCGGTACCCCTCCTCAAGAGGTAGTTCCGGAATCCCTCCCCACCCCGGATGAGCCCGTACAATTCCCGAAAGA
AGCAACCGGTAAACCTGAAGAGCTGGATACTTTCGATGAGCTTCTCGAAGATGTCCCCCCGGCGCAAGAAGGTGTAGAAC
CCCCGGTACTAAGCGGGGACGATGTTCGCGCGGCACTGGGCAAGATCATGGGACAACCGGAGATGGTCCCCCCGTCGGCG
CACTCCCCGGAGCCTCTTACAGCCTCCCCGGAATCAGAGTTACCCCCGGAACAAGTTGTCGAAATTACTGAAGAGGAAGA
TCCCCGCGAGAATCGCACGTCCCGGTTTTTCAAGAAAAAGAAGGCCGAAAAAGTGGGAGCCCCGACAGATCAGATCGGGA
AATCCGCGGTGACCGAATCTGATGAGCGGATTATTTCCGCTGACCAGATATCCGAATTTTCCGGTCTTATTCTCCCCAAA
GGTGCGACATTCCATGTTGATGAGATCAATCTTCATGGCCGGATGCAGGCGTTTGAGTCCACCGGAACCGGTAGCCTTCC
CCCGGAACTTGCCGAGATCTGGAAAAGGGAGTTTTCCACATCCGGTTTCAAGGACCTTGAATCAGAACTGACCCTTGCAG
ACAAGGGGACGGATAAGGGGACAGAAAAGAAATCCTGGTTTAGTGCACTCTTCAGCGCGATCCGGTCGGAGAACGTGGAA
TACGATCCCAAGATCCATGGACCTCTCGTCGATCTCACCTTTGAGCCAAAACCCGGTATCGAAGTGATGGATACGTACCC
CGTCAACGATCCCTATGCATATGTCCGGGTCATCTATGACCATTCCACCCATGAATATACCTACCAGGTTCTCGAACCTG
TACTCTCGGAGCCAGAGAAAGACCTGTTAAAGGAGTTAAAGGAACGCCTTTTTGAGATCCTTGACATCAACACCAAGGAC
ATTTCCAAAGAGGAGGCCCGCAGCCAGCTCCGTCGTTCGGTTGATGAGATTCTGGCGGATTACGGAATCAAGCTCAACCC
GGTGGAACGTGAGAAGATCCTCTATAACATGCACAAGGACTTTCTGGGTGATGGCATGATCGATGCGATCATGCATGACA
AGTATATCGAGGATATCTCCTGCGACGGGGTCAACACCCCTCTCTTTGCCTTCCATGCCAATTACGAGTCAATGAAGACC
ACCCTGATGTACCACAATGCCGAGGAGCTCGACTCCTTTGTCACCAAGCTCGCACAAAGGGCAGGCAAGTACATCTCCAT
TGCCGAGCCCATTCTCGATGCGACCATGCAGGATGGATCCCGTATCCAGATGACACTGGGGCAGGAGGTCACTGCGCACG
GCTCGACATTCACTATCCGTAAGTTCAAGGACGAGCCAATTACCCCTACCGATCTCATTGAATGGCACACCTTTGCCCCG
CTCTCGATCGCTTACATATGGCTCTGCGTCGAGAATGGCAAGTCCGCTATCTTTGCCGGTGGGACCGCGTCCGGGAAGAC
TACTGCCCTCAATGCAATCTCGCTTTTCATTCCCCCGATGGCAAAGATTGTCTCTCTGGAGGATACCCGTGAAGTCAAGC
TTCCTCACCCGAACTGGATCCCGAGTGTCACGCGTGACTCATTTGACACCGCCGGCAGAGGAGAGATCAACATGTATGAG
TTGCTCCGTGCGGCTATGCGTCAGCGGCCCGAGTACATCATCGTCGGTGAGGTTAGAGGTAAGGAATGCCAGACACTTTT
CCAGGCAATGAGCACGGGCCACGTTACCTACTCCACAACTCACGCCGACTCTGTTGCGAGCGTCGTGCACCGTATCGAGA
ACCCGCCCATGGACGTACCACGGAATATGCTCTCCGCGCTCGATTTCATCTGTGTCCAGGTACAGGCCCGGATGGGCGGG
AAACGGATCCGCCGGAACAAGCAGATTGTTGAGATCCTCGATATCGATCCGCGGACAAACGAACTGATCACAAACGAGGT
CTTCCGCTGGCGATCGGCAACCGATGAGATCACCTACTCGGGGAAATCTTACATCCTTGAGGACATCATGGAGGCGCGGG
GCTGGAACGAGAACCGTATGCGGGAAGAACTCAAACGCAGGCAGGAGGTGCTGGAGTGGATGCGTATCAAGAAGATCCGG
CACTACAAGGATGTAGCAAAGATCCTTATCTCGTACTTCCGTGAGCCCGAAGTGGTTGTAGAGCGCGTAAGGAAGGATCT
ATATGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(400-626)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7I9L0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdL Methanoculleus thermophilus strain DSM 2373

66.886

81.041

0.542

Multiple sequence alignment