Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SHEW185_RS15755 Genome accession   NC_009665
Coordinates   3631786..3632898 (-) Length   370 a.a.
NCBI ID   WP_006085705.1    Uniprot ID   -
Organism   Shewanella baltica OS185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3626786..3637898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEW185_RS15720 (Shew185_3031) - 3627274..3627834 (-) 561 WP_012089749.1 DUF4136 domain-containing protein -
  SHEW185_RS15725 (Shew185_3032) - 3628014..3628331 (+) 318 WP_012089750.1 hypothetical protein -
  SHEW185_RS15730 (Shew185_3033) yciH 3628510..3628839 (+) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  SHEW185_RS15735 (Shew185_3034) - 3628906..3629469 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  SHEW185_RS15740 (Shew185_3035) ruvX 3629499..3629924 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  SHEW185_RS15745 (Shew185_3036) hemH 3630013..3631026 (-) 1014 WP_012089751.1 ferrochelatase -
  SHEW185_RS15750 (Shew185_3037) - 3631059..3631598 (-) 540 WP_037392614.1 glutathione peroxidase -
  SHEW185_RS15755 (Shew185_3038) pilU 3631786..3632898 (-) 1113 WP_006085705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEW185_RS15760 (Shew185_3039) pilT 3632908..3633945 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEW185_RS15765 (Shew185_3040) - 3633981..3634679 (+) 699 WP_012089753.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEW185_RS15770 (Shew185_3041) proC 3634895..3635713 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  SHEW185_RS15775 (Shew185_3042) - 3635762..3636310 (+) 549 WP_006082520.1 YggT family protein -
  SHEW185_RS15780 (Shew185_3043) yggU 3636310..3636609 (+) 300 WP_006082521.1 DUF167 family protein YggU -
  SHEW185_RS15785 (Shew185_3044) - 3636741..3637175 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40986.13 Da        Isoelectric Point: 6.2352

>NTDB_id=28601 SHEW185_RS15755 WP_006085705.1 3631786..3632898(-) (pilU) [Shewanella baltica OS185]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=28601 SHEW185_RS15755 WP_006085705.1 3631786..3632898(-) (pilU) [Shewanella baltica OS185]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTTATCACAGCTGGCTTTCCGCCGAG
TGCCAAAATTGACGGTGAGTTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCGACTCGTGAGTGTAACTTTGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGTGCATTTTGGCAGCGTGAATCGCCAGGCTGCGTGATGCGCCGTATTGAGACTAAAATCCCTGACGTCGA
TGATTTAAAACTGCCCCCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGTCTCATTATTATGGTGGGCGGAACGGGTA
CGGGTAAGTCGACATCCCTAGCGGCCTTGGTGGGGTATCGAAATGGTCATGCTCGTGGTCATATTCTCACGATTGAAGAT
CCGGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAGTCTTTCGATGC
AGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CCCTGTCGTTTGCGGAAACGGGTCATCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAGCAGTTATTGTTCGATTTGTCATTGAATTTGCGCGGTATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGCCGCGCCGCGATTGAAGTATTGATCAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTGCTCAAAGAAACCATGGCTAAATCGAATGAGCAGGGCATGCAGACGTTCGACCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAATTACAGAATAAAGAATCCACGGGCTCAGGCTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment