Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SHEW185_RS15760 Genome accession   NC_009665
Coordinates   3632908..3633945 (-) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella baltica OS185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3627908..3638945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEW185_RS15725 (Shew185_3032) - 3628014..3628331 (+) 318 WP_012089750.1 hypothetical protein -
  SHEW185_RS15730 (Shew185_3033) yciH 3628510..3628839 (+) 330 WP_037392612.1 stress response translation initiation inhibitor YciH -
  SHEW185_RS15735 (Shew185_3034) - 3628906..3629469 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  SHEW185_RS15740 (Shew185_3035) ruvX 3629499..3629924 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  SHEW185_RS15745 (Shew185_3036) hemH 3630013..3631026 (-) 1014 WP_012089751.1 ferrochelatase -
  SHEW185_RS15750 (Shew185_3037) - 3631059..3631598 (-) 540 WP_037392614.1 glutathione peroxidase -
  SHEW185_RS15755 (Shew185_3038) pilU 3631786..3632898 (-) 1113 WP_006085705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SHEW185_RS15760 (Shew185_3039) pilT 3632908..3633945 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  SHEW185_RS15765 (Shew185_3040) - 3633981..3634679 (+) 699 WP_012089753.1 YggS family pyridoxal phosphate-dependent enzyme -
  SHEW185_RS15770 (Shew185_3041) proC 3634895..3635713 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  SHEW185_RS15775 (Shew185_3042) - 3635762..3636310 (+) 549 WP_006082520.1 YggT family protein -
  SHEW185_RS15780 (Shew185_3043) yggU 3636310..3636609 (+) 300 WP_006082521.1 DUF167 family protein YggU -
  SHEW185_RS15785 (Shew185_3044) - 3636741..3637175 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -
  SHEW185_RS15790 (Shew185_3045) rdgB 3637315..3637932 (+) 618 WP_012089754.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=28602 SHEW185_RS15760 WP_006082517.1 3632908..3633945(-) (pilT) [Shewanella baltica OS185]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=28602 SHEW185_RS15760 WP_006082517.1 3632908..3633945(-) (pilT) [Shewanella baltica OS185]
ATGGAAATCACTGAGTTATTAGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAGATTAACCTGCCCGCGCTCGATCACCAAGGCGTACATAGCCTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCGCGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCTGCGGCCGTATTTCGTACCATTCCTAGCGACATCTTGTCACTTGA
GCAATTAGGTGCGCCGGAGATTTTTAAAAAAATCGCCAGTTTCCCTCGGGGATTAGTCCTAGTGACTGGACCAACGGGTT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTTAACGAAAATCGACACGATCACATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAAAACAAGCAATGTTTGATTAACCAGCGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGATCCGGACGTGATTTTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCCGCAGAAACAGGCCACTTAGTGTTTGGTACTTTGCATACCACCTCCGCCGCGAAAACCATTGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAAAAAGACATGGTACGCACTATGTTGTCGGAATCGCTGCAAGCGGTTATTTCGCAAAC
ACTGATTAAAAAGGTTGGTGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACTCCCGCTATCCGTAACCTTATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCGATTCAAACCGGTATGGCCCACGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACCCGTGAAGATGCAATGGCGAAGAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment