Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CDG55_RS15150 Genome accession   NZ_CP028561
Coordinates   2870154..2871335 (+) Length   393 a.a.
NCBI ID   WP_005158869.1    Uniprot ID   N9KH47
Organism   Acinetobacter sp. WCHA45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2865154..2876335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG55_RS15125 (CDG55_15115) - 2865801..2866274 (-) 474 WP_087537324.1 hypothetical protein -
  CDG55_RS15130 (CDG55_15120) guaD 2866461..2867777 (+) 1317 WP_087537325.1 guanine deaminase -
  CDG55_RS15135 (CDG55_15125) hpt 2868002..2868529 (+) 528 WP_087537326.1 hypoxanthine phosphoribosyltransferase -
  CDG55_RS15140 (CDG55_15130) - 2868702..2868953 (+) 252 WP_004660536.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  CDG55_RS15145 (CDG55_15135) - 2869013..2869711 (-) 699 WP_087537327.1 DsbC family protein -
  CDG55_RS15150 (CDG55_15140) pilU 2870154..2871335 (+) 1182 WP_005158869.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG55_RS15155 (CDG55_15145) - 2871409..2871744 (-) 336 WP_228252561.1 hypothetical protein -
  CDG55_RS15160 (CDG55_15150) mnmE 2871994..2873349 (-) 1356 WP_087537329.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  CDG55_RS15165 (CDG55_15155) yidC 2873464..2875224 (-) 1761 WP_087537330.1 membrane protein insertase YidC -
  CDG55_RS15170 (CDG55_15160) yidD 2875230..2875550 (-) 321 WP_087537331.1 membrane protein insertion efficiency factor YidD -
  CDG55_RS15175 (CDG55_15165) rnpA 2875560..2875955 (-) 396 WP_087537332.1 ribonuclease P protein component -
  CDG55_RS15180 (CDG55_15170) rpmH 2875983..2876117 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 44641.22 Da        Isoelectric Point: 6.4457

>NTDB_id=285535 CDG55_RS15150 WP_005158869.1 2870154..2871335(+) (pilU) [Acinetobacter sp. WCHA45]
MYNAELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQTLTPEKTKLFAYSLMNEKQRQEFENEWEC
NFAINIPEVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVMMEKRGLILVVGSTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIMNFFPDERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAVEILLNTPLIAENILKGQFHNLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSLNELRLQIKLKSTRQEGVPASAATSLNMLKEDSEEQEMQGI

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=285535 CDG55_RS15150 WP_005158869.1 2870154..2871335(+) (pilU) [Acinetobacter sp. WCHA45]
ATGTATAATGCGGAATTATTAGAAGAAGCCAAAACGTTTATGTTTCATATGTTGACCAAAGTGGTTGAATATGGCGGCTC
AGATTTATTCATTACAGCAGACTTTCCACCGAGTATTAAACTCCAAGGTTTAATGCGTCCTTTAGGTCAGCAAACTTTAA
CCCCAGAAAAAACTAAGCTATTTGCATATAGCTTAATGAATGAAAAGCAACGCCAAGAATTTGAAAATGAATGGGAATGT
AATTTCGCAATTAATATTCCAGAAGTATCACGCTTTCGTGTCAATGTATTTAAGCAACAACTACAAATCGGTATGGTTAT
CCGAACCATTACGTCAGAAATCCCAACCTTCCAAAAGCTCAAACTCCCAGAATCATTGAAAAATGTCATGATGGAAAAAC
GTGGCCTAATATTGGTAGTTGGAAGTACGGGATCAGGTAAATCTACGTCATTGGCAGCCATGATTGACCATCGTAATGAA
AATTCGGCGGGGCATATTATTACTGTTGAAGACCCCGTGGAATATGTCCATAAGCATAAAAAGTCAATGATTACCCACCG
AGAAGTCGGTGTAGATTGCCATACTTGGCACCATGCCTTAAAAAATACATTGCGTCAGGCACCTGATGTCATTTTGATTG
GTGAAATCCGTGATACTGAAACCATGGAACATGCCATTGCTTTTGCTGAAACTGGGCATTTATGTTTAGGAACTTTACAT
GCCAACAACGCCAATCAAGCATTAGATCGGATCATGAACTTCTTTCCAGATGAACGCCGTAATCAATTGCTGATGGATCT
TTCATCTAATATGAAAGCGATTATTTCTCAACGCTTGGTTCGTACTCAAGATGGTCAAGGTCGTCGTGCAGCCGTAGAGA
TTCTACTGAATACACCATTAATTGCAGAAAATATCTTAAAAGGTCAATTTCATAATCTTAAAGAAATTATGACCAAGTCT
CGTGAATTAGGTATGCAAACTTTTGATCAGGCCTTGTTTGATTTATACAACGAAGGTGCAATTAGTTATGATGAAGCGTT
ACGTAATGCTGACTCATTGAATGAGTTACGCTTACAAATTAAGCTGAAAAGTACTCGTCAAGAAGGAGTTCCAGCTAGTG
CAGCAACTTCTTTAAATATGCTGAAAGAAGACTCTGAAGAGCAAGAAATGCAAGGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9KH47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.77

88.55

0.529
  pilU Acinetobacter baylyi ADP1

56.196

88.295

0.496
  pilU Vibrio cholerae strain A1552

51.445

88.041

0.453

Multiple sequence alignment