Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DA378_RS19170 Genome accession   NZ_CP028440
Coordinates   3835451..3836521 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain J7-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3830451..3841521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA378_RS19140 - 3830926..3831171 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  DA378_RS19145 - 3831408..3831788 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DA378_RS19155 - 3831994..3832824 (+) 831 WP_003153080.1 STAS domain-containing protein -
  DA378_RS19160 - 3832862..3834163 (-) 1302 WP_003153081.1 hemolysin family protein -
  DA378_RS19165 - 3834309..3835259 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  DA378_RS19170 comGA 3835451..3836521 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  DA378_RS19175 comGB 3836508..3837545 (+) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  DA378_RS19180 comGC 3837592..3837858 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  DA378_RS19185 comGD 3837848..3838285 (+) 438 WP_003153088.1 competence type IV pilus minor pilin ComGD Machinery gene
  DA378_RS19190 comGE 3838269..3838583 (+) 315 WP_003153089.1 competence type IV pilus minor pilin ComGE -
  DA378_RS19885 comGF 3838776..3838991 (+) 216 WP_254051908.1 ComGF family competence protein Machinery gene
  DA378_RS19200 comGG 3838992..3839369 (+) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  DA378_RS19205 - 3839426..3839605 (+) 180 WP_003153093.1 YqzE family protein -
  DA378_RS19210 - 3839645..3839974 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  DA378_RS19215 tapA 3840233..3840904 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  DA378_RS19220 sipW 3840876..3841460 (+) 585 WP_003153100.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=285037 DA378_RS19170 WP_003153083.1 3835451..3836521(+) (comGA) [Bacillus velezensis strain J7-1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=285037 DA378_RS19170 WP_003153083.1 3835451..3836521(+) (comGA) [Bacillus velezensis strain J7-1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment