Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   DA377_RS08645 Genome accession   NZ_CP028439
Coordinates   1632148..1633185 (+) Length   345 a.a.
NCBI ID   WP_003153086.1    Uniprot ID   -
Organism   Bacillus velezensis strain 8-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1627148..1638185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA377_RS08625 - 1627634..1628464 (+) 831 WP_003153080.1 STAS domain-containing protein -
  DA377_RS08630 - 1628502..1629803 (-) 1302 WP_003153081.1 hemolysin family protein -
  DA377_RS08635 - 1629949..1630899 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  DA377_RS08640 comGA 1631091..1632161 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  DA377_RS08645 comGB 1632148..1633185 (+) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  DA377_RS08650 comGC 1633232..1633498 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  DA377_RS08655 comGD 1633488..1633925 (+) 438 WP_003153088.1 competence type IV pilus minor pilin ComGD Machinery gene
  DA377_RS08660 comGE 1633909..1634223 (+) 315 WP_003153089.1 competence type IV pilus minor pilin ComGE -
  DA377_RS08665 comGF 1634132..1634632 (+) 501 WP_223203779.1 competence type IV pilus minor pilin ComGF -
  DA377_RS08670 comGG 1634633..1635010 (+) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  DA377_RS08675 - 1635067..1635246 (+) 180 WP_003153093.1 YqzE family protein -
  DA377_RS08680 - 1635286..1635615 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  DA377_RS08685 tapA 1635874..1636545 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  DA377_RS08690 sipW 1636517..1637101 (+) 585 WP_003153100.1 signal peptidase I SipW -
  DA377_RS08695 tasA 1637165..1637950 (+) 786 WP_003153102.1 biofilm matrix protein TasA -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39544.84 Da        Isoelectric Point: 9.4989

>NTDB_id=284929 DA377_RS08645 WP_003153086.1 1632148..1633185(+) (comGB) [Bacillus velezensis strain 8-2]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=284929 DA377_RS08645 WP_003153086.1 1632148..1633185(+) (comGB) [Bacillus velezensis strain 8-2]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCAGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGATTTGTTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.325

93.623

0.612

Multiple sequence alignment