Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   C9I44_RS04460 Genome accession   NZ_CP028386
Coordinates   866714..867970 (-) Length   418 a.a.
NCBI ID   WP_004257939.1    Uniprot ID   -
Organism   Lactococcus garvieae strain IBB3403     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861714..872970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9I44_RS04425 (C9I44_04375) rpmA 861834..862118 (+) 285 WP_004257956.1 50S ribosomal protein L27 -
  C9I44_RS04430 (C9I44_04380) - 862470..863575 (+) 1106 Protein_867 alanine dehydrogenase -
  C9I44_RS04435 (C9I44_04385) - 863693..864652 (+) 960 WP_004257953.1 PhoH family protein -
  C9I44_RS04440 (C9I44_04390) - 864654..865127 (+) 474 WP_004257950.1 NUDIX hydrolase -
  C9I44_RS04445 (C9I44_04395) ybeY 865137..865625 (+) 489 WP_004257946.1 rRNA maturation RNase YbeY -
  C9I44_RS04450 (C9I44_04400) - 865609..866070 (+) 462 WP_004257944.1 diacylglycerol kinase family protein -
  C9I44_RS04455 (C9I44_04405) - 866073..866717 (-) 645 WP_004257941.1 ComF family protein -
  C9I44_RS04460 (C9I44_04410) comFA 866714..867970 (-) 1257 WP_004257939.1 DEAD/DEAH box helicase Machinery gene
  C9I44_RS04465 (C9I44_04415) - 868025..868648 (+) 624 WP_017370876.1 YigZ family protein -
  C9I44_RS04470 (C9I44_04420) - 868662..869684 (+) 1023 WP_004257934.1 DUF475 domain-containing protein -
  C9I44_RS04475 (C9I44_04425) - 869714..870736 (-) 1023 WP_004257931.1 alpha/beta hydrolase -
  C9I44_RS04480 (C9I44_04430) - 871055..871834 (+) 780 WP_004257929.1 formate/nitrite transporter family protein -
  C9I44_RS04485 (C9I44_04435) glyQ 871848..872816 (+) 969 WP_004257927.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47861.62 Da        Isoelectric Point: 9.9268

>NTDB_id=284576 C9I44_RS04460 WP_004257939.1 866714..867970(-) (comFA) [Lactococcus garvieae strain IBB3403]
MDELYGRLLLQKELTKLPDKAILFDGMQDVSQTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWQGKLTASQQHVSDSLVNLHQQQKTVLVQAVTGAGKTEMIYQSINNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATAHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALTQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=284576 C9I44_RS04460 WP_004257939.1 866714..867970(-) (comFA) [Lactococcus garvieae strain IBB3403]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAATACTTTTTGATGGGAT
GCAAGATGTTTCCCAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATTAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTTCGCTCGGACGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAATTT
GCATCAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACTGAAATGATTTATCAGAGCATTAATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCACCACTCGTAATTGCAACGGC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTTGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCGCTGACTCAAGCCCGTAAACCTTCTTCAAGCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAGAAGCTACAGCTCCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATTTAGGAAAAACTACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGAGGGTTCTTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(92-236)

Helicase conserved C-terminal domain (Helicase_C)

(274-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.143

95.455

0.545
  comFA/cflA Streptococcus mitis SK321

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae TIGR4

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae Rx1

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae D39

51.878

100

0.529
  comFA/cflA Streptococcus pneumoniae R6

51.878

100

0.529
  comFA/cflA Streptococcus mitis NCTC 12261

50.939

100

0.519
  comFA Bacillus subtilis subsp. subtilis str. 168

38.791

94.976

0.368
  comFA Latilactobacillus sakei subsp. sakei 23K

38.287

94.976

0.364

Multiple sequence alignment