Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EGX93_RS23375 Genome accession   NZ_CP033762
Coordinates   4560177..4560617 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain FDAARGOS_536     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4555177..4565617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGX93_RS23355 (EGX93_23365) coaE 4555667..4556287 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  EGX93_RS25905 - 4556312..4556356 (+) 45 WP_120795372.1 protein YacM -
  EGX93_RS23360 (EGX93_23370) guaC 4556512..4557555 (+) 1044 WP_001217338.1 GMP reductase -
  EGX93_RS23365 (EGX93_23375) hofC 4557590..4558792 (-) 1203 WP_001540700.1 protein transport protein HofC -
  EGX93_RS23370 (EGX93_23380) pilB 4558782..4560167 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  EGX93_RS23375 (EGX93_23385) pilA 4560177..4560617 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  EGX93_RS23380 (EGX93_23390) nadC 4560821..4561714 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGX93_RS23385 (EGX93_23395) ampD 4561802..4562353 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EGX93_RS23390 (EGX93_23400) ampE 4562350..4563204 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  EGX93_RS23395 (EGX93_23405) aroP 4563247..4564617 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=284477 EGX93_RS23375 WP_000360914.1 4560177..4560617(-) (pilA) [Escherichia coli strain FDAARGOS_536]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=284477 EGX93_RS23375 WP_000360914.1 4560177..4560617(-) (pilA) [Escherichia coli strain FDAARGOS_536]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment