Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C7M27_RS05235 Genome accession   NZ_CP028213
Coordinates   1027865..1028482 (+) Length   205 a.a.
NCBI ID   WP_103804084.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM102749     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022865..1033482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M27_RS05200 (C7M27_01022) aroE 1023523..1024365 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  C7M27_RS05205 (C7M27_01023) yhbY 1024359..1024649 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C7M27_RS05210 (C7M27_01024) nadD 1024661..1025230 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  C7M27_RS05215 (C7M27_01025) yqeK 1025220..1025780 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C7M27_RS05220 (C7M27_01026) rsfS 1025798..1026154 (+) 357 WP_014480315.1 ribosome silencing factor -
  C7M27_RS05225 (C7M27_01027) yqeM 1026151..1026894 (+) 744 WP_032726197.1 class I SAM-dependent methyltransferase -
  C7M27_RS05230 (C7M27_01028) comER 1026960..1027781 (-) 822 WP_032726195.1 late competence protein ComER -
  C7M27_RS05235 (C7M27_01029) comEA 1027865..1028482 (+) 618 WP_103804084.1 competence protein ComEA Machinery gene
  C7M27_RS05240 (C7M27_01030) comEB 1028550..1029119 (+) 570 WP_003229978.1 ComE operon protein 2 -
  C7M27_RS05245 (C7M27_01031) comEC 1029123..1031453 (+) 2331 WP_128740584.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C7M27_RS05250 (C7M27_01032) yqzM 1031494..1031628 (-) 135 WP_003229983.1 YqzM family protein -
  C7M27_RS05255 - 1031705..1031818 (+) 114 WP_122060479.1 hypothetical protein -
  C7M27_RS05260 (C7M27_01033) holA 1031858..1032901 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  C7M27_RS05265 (C7M27_01034) rpsT 1032916..1033182 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21737.54 Da        Isoelectric Point: 4.9680

>NTDB_id=283165 C7M27_RS05235 WP_103804084.1 1027865..1028482(+) (comEA) [Bacillus subtilis strain SRCM102749]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSVGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=283165 C7M27_RS05235 WP_103804084.1 1027865..1028482(+) (comEA) [Bacillus subtilis strain SRCM102749]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGTTGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.073

100

0.971
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment