Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C7M23_RS11585 Genome accession   NZ_CP028209
Coordinates   2225391..2226008 (+) Length   205 a.a.
NCBI ID   WP_131227639.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM102745     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2220391..2231008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M23_RS11550 (C7M23_02257) aroE 2221049..2221891 (+) 843 WP_131227643.1 shikimate dehydrogenase -
  C7M23_RS11555 (C7M23_02258) yhbY 2221885..2222175 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C7M23_RS11560 (C7M23_02259) nadD 2222187..2222756 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  C7M23_RS11565 (C7M23_02260) yqeK 2222746..2223306 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C7M23_RS11570 (C7M23_02261) rsfS 2223324..2223680 (+) 357 WP_131227641.1 ribosome silencing factor -
  C7M23_RS11575 (C7M23_02262) yqeM 2223677..2224420 (+) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  C7M23_RS11580 (C7M23_02263) comER 2224486..2225307 (-) 822 WP_014477389.1 late competence protein ComER -
  C7M23_RS11585 (C7M23_02264) comEA 2225391..2226008 (+) 618 WP_131227639.1 competence protein ComEA Machinery gene
  C7M23_RS11590 (C7M23_02265) comEB 2226075..2226644 (+) 570 WP_003229978.1 ComE operon protein 2 -
  C7M23_RS11595 (C7M23_02266) comEC 2226648..2228978 (+) 2331 WP_042976534.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C7M23_RS11600 (C7M23_02267) yqzM 2229018..2229152 (-) 135 WP_003229983.1 YqzM family protein -
  C7M23_RS11605 - 2229193..2229342 (+) 150 WP_003229985.1 hypothetical protein -
  C7M23_RS11610 (C7M23_02268) holA 2229382..2230425 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  C7M23_RS11615 (C7M23_02269) rpsT 2230440..2230706 (-) 267 WP_131227637.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21795.53 Da        Isoelectric Point: 4.7220

>NTDB_id=282874 C7M23_RS11585 WP_131227639.1 2225391..2226008(+) (comEA) [Bacillus subtilis strain SRCM102745]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=282874 C7M23_RS11585 WP_131227639.1 2225391..2226008(+) (comEA) [Bacillus subtilis strain SRCM102745]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGTGGAGGGTCTG
TCCAAAGTGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

35.909

100

0.385

Multiple sequence alignment