Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   C7M22_RS19935 Genome accession   NZ_CP028208
Coordinates   4022753..4023823 (-) Length   356 a.a.
NCBI ID   WP_043020785.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM102744     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4017753..4028823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M22_RS19885 (C7M22_04011) sipW 4017813..4018397 (-) 585 WP_160223172.1 signal peptidase I SipW -
  C7M22_RS19890 (C7M22_04012) tapA 4018369..4019040 (-) 672 WP_160223173.1 amyloid fiber anchoring/assembly protein TapA -
  C7M22_RS19895 (C7M22_04013) - 4019299..4019628 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  C7M22_RS19900 (C7M22_04014) - 4019668..4019847 (-) 180 WP_160223174.1 YqzE family protein -
  C7M22_RS19905 (C7M22_04015) comGG 4019904..4020281 (-) 378 WP_160223175.1 competence type IV pilus minor pilin ComGG Machinery gene
  C7M22_RS19910 (C7M22_04016) comGF 4020282..4020782 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  C7M22_RS19915 (C7M22_04017) comGE 4020691..4021005 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  C7M22_RS19920 (C7M22_04018) comGD 4020989..4021426 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  C7M22_RS19925 (C7M22_04019) comGC 4021416..4021682 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  C7M22_RS19930 (C7M22_04020) comGB 4021729..4022766 (-) 1038 WP_061582071.1 competence type IV pilus assembly protein ComGB Machinery gene
  C7M22_RS19935 (C7M22_04021) comGA 4022753..4023823 (-) 1071 WP_043020785.1 competence type IV pilus ATPase ComGA Machinery gene
  C7M22_RS19940 (C7M22_04022) - 4024020..4024970 (-) 951 WP_160223176.1 magnesium transporter CorA family protein -
  C7M22_RS19945 (C7M22_04023) - 4025116..4026417 (+) 1302 WP_012117986.1 hemolysin family protein -
  C7M22_RS19950 (C7M22_04024) - 4026455..4027285 (-) 831 WP_015240214.1 STAS domain-containing protein -
  C7M22_RS19960 (C7M22_04026) - 4027492..4027872 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  C7M22_RS19965 (C7M22_04027) - 4028109..4028354 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=282831 C7M22_RS19935 WP_043020785.1 4022753..4023823(-) (comGA) [Bacillus velezensis strain SRCM102744]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTQRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=282831 C7M22_RS19935 WP_043020785.1 4022753..4023823(-) (comGA) [Bacillus velezensis strain SRCM102744]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGGCACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACAACGTGCAGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379

Multiple sequence alignment