Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C7M16_RS11630 Genome accession   NZ_CP028201
Coordinates   2319791..2320408 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM102753     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2314791..2325408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M16_RS11600 (C7M16_02293) rpsT 2315093..2315359 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  C7M16_RS11605 (C7M16_02294) holA 2315374..2316417 (-) 1044 WP_160221684.1 DNA polymerase III subunit delta -
  C7M16_RS11610 - 2316457..2316606 (-) 150 WP_003229985.1 hypothetical protein -
  C7M16_RS11615 (C7M16_02295) yqzM 2316647..2316781 (+) 135 WP_003229983.1 YqzM family protein -
  C7M16_RS11620 (C7M16_02296) comEC 2316821..2319151 (-) 2331 WP_046381195.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C7M16_RS11625 (C7M16_02297) comEB 2319155..2319724 (-) 570 WP_003229978.1 ComE operon protein 2 -
  C7M16_RS11630 (C7M16_02298) comEA 2319791..2320408 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  C7M16_RS11635 (C7M16_02299) comER 2320492..2321313 (+) 822 WP_014480313.1 late competence protein ComER -
  C7M16_RS11640 (C7M16_02300) yqeM 2321379..2322122 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  C7M16_RS11645 (C7M16_02301) rsfS 2322119..2322475 (-) 357 WP_041333759.1 ribosome silencing factor -
  C7M16_RS11650 (C7M16_02302) yqeK 2322493..2323053 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C7M16_RS11655 (C7M16_02303) nadD 2323043..2323612 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  C7M16_RS11660 (C7M16_02304) yhbY 2323624..2323914 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C7M16_RS11665 (C7M16_02305) aroE 2323908..2324750 (-) 843 WP_029317883.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=282345 C7M16_RS11630 WP_014480312.1 2319791..2320408(-) (comEA) [Bacillus subtilis strain SRCM102753]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=282345 C7M16_RS11630 WP_014480312.1 2319791..2320408(-) (comEA) [Bacillus subtilis strain SRCM102753]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment