Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   C9J86_RS12760 Genome accession   NZ_CP028190
Coordinates   2509476..2509973 (-) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CFSAN018749     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2504476..2514973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9J86_RS12735 (C9J86_12735) gcvPB 2504638..2506110 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  C9J86_RS12740 (C9J86_12740) gcvPA 2506103..2507449 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  C9J86_RS12745 (C9J86_12745) gcvT 2507469..2508560 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  C9J86_RS12750 (C9J86_12750) - 2508719..2509243 (-) 525 WP_001015125.1 shikimate kinase -
  C9J86_RS12755 (C9J86_12755) - 2509233..2509379 (-) 147 WP_001789879.1 hypothetical protein -
  C9J86_RS12760 (C9J86_12760) comGF 2509476..2509973 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  C9J86_RS12765 (C9J86_12765) comGE 2509891..2510190 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  C9J86_RS12770 (C9J86_12770) comGD 2510177..2510623 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  C9J86_RS12775 (C9J86_12775) comGC 2510601..2510912 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  C9J86_RS12780 (C9J86_12780) comGB 2510926..2511996 (-) 1071 WP_053865724.1 competence type IV pilus assembly protein ComGB Machinery gene
  C9J86_RS12785 (C9J86_12785) comGA 2511968..2512942 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  C9J86_RS12790 (C9J86_12790) - 2512994..2513617 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  C9J86_RS12795 (C9J86_12795) - 2513614..2513943 (-) 330 WP_053865723.1 MTH1187 family thiamine-binding protein -
  C9J86_RS12800 (C9J86_12800) - 2513943..2514929 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=282214 C9J86_RS12760 WP_001794328.1 2509476..2509973(-) (comGF) [Staphylococcus aureus strain CFSAN018749]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=282214 C9J86_RS12760 WP_001794328.1 2509476..2509973(-) (comGF) [Staphylococcus aureus strain CFSAN018749]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment