Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   C9J86_RS12785 Genome accession   NZ_CP028190
Coordinates   2511968..2512942 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain CFSAN018749     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2506968..2517942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9J86_RS12745 (C9J86_12745) gcvT 2507469..2508560 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  C9J86_RS12750 (C9J86_12750) - 2508719..2509243 (-) 525 WP_001015125.1 shikimate kinase -
  C9J86_RS12755 (C9J86_12755) - 2509233..2509379 (-) 147 WP_001789879.1 hypothetical protein -
  C9J86_RS12760 (C9J86_12760) comGF 2509476..2509973 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  C9J86_RS12765 (C9J86_12765) comGE 2509891..2510190 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  C9J86_RS12770 (C9J86_12770) comGD 2510177..2510623 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  C9J86_RS12775 (C9J86_12775) comGC 2510601..2510912 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  C9J86_RS12780 (C9J86_12780) comGB 2510926..2511996 (-) 1071 WP_053865724.1 competence type IV pilus assembly protein ComGB Machinery gene
  C9J86_RS12785 (C9J86_12785) comGA 2511968..2512942 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  C9J86_RS12790 (C9J86_12790) - 2512994..2513617 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  C9J86_RS12795 (C9J86_12795) - 2513614..2513943 (-) 330 WP_053865723.1 MTH1187 family thiamine-binding protein -
  C9J86_RS12800 (C9J86_12800) - 2513943..2514929 (-) 987 WP_000161314.1 ROK family glucokinase -
  C9J86_RS12805 (C9J86_12805) - 2514926..2515129 (-) 204 WP_000087561.1 YqgQ family protein -
  C9J86_RS12810 (C9J86_12810) - 2515110..2516573 (-) 1464 WP_001017867.1 rhomboid family protein -
  C9J86_RS12815 (C9J86_12815) - 2516585..2517124 (-) 540 WP_000164553.1 5-formyltetrahydrofolate cyclo-ligase -
  C9J86_RS12820 (C9J86_12820) rpmG 2517333..2517482 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=282219 C9J86_RS12785 WP_000697220.1 2511968..2512942(-) (comGA) [Staphylococcus aureus strain CFSAN018749]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=282219 C9J86_RS12785 WP_000697220.1 2511968..2512942(-) (comGA) [Staphylococcus aureus strain CFSAN018749]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTGCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment