Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LL14B4_RS05360 Genome accession   NZ_CP028160
Coordinates   1084276..1085598 (-) Length   440 a.a.
NCBI ID   WP_109990846.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain 14B4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1079276..1090598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL14B4_RS05330 (LL14B4_05325) - 1080419..1081387 (+) 969 WP_003130649.1 PhoH family protein -
  LL14B4_RS05335 (LL14B4_05330) - 1081445..1081924 (+) 480 WP_021722517.1 NUDIX hydrolase -
  LL14B4_RS05340 (LL14B4_05335) ybeY 1082076..1082564 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  LL14B4_RS05345 (LL14B4_05340) - 1082548..1083003 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  LL14B4_RS05350 (LL14B4_05345) - 1083017..1083595 (+) 579 WP_021722515.1 nucleotidyltransferase family protein -
  LL14B4_RS13695 (LL14B4_05350) - 1083629..1084279 (-) 651 WP_109990845.1 ComF family protein -
  LL14B4_RS05360 (LL14B4_05355) comFA 1084276..1085598 (-) 1323 WP_109990846.1 DEAD/DEAH box helicase Machinery gene
  LL14B4_RS05365 (LL14B4_05360) - 1085655..1086284 (+) 630 WP_003130660.1 YigZ family protein -
  LL14B4_RS05370 (LL14B4_05365) - 1086418..1086915 (+) 498 WP_003130662.1 VanZ family protein -
  LL14B4_RS05375 (LL14B4_05370) - 1086963..1087982 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  LL14B4_RS05380 (LL14B4_05375) - 1088097..1088843 (+) 747 WP_200136626.1 bifunctional metallophosphatase/5'-nucleotidase -
  LL14B4_RS05385 (LL14B4_05380) glyQ 1089130..1090083 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50234.79 Da        Isoelectric Point: 10.1065

>NTDB_id=281806 LL14B4_RS05360 WP_109990846.1 1084276..1085598(-) (comFA) [Lactococcus lactis subsp. lactis strain 14B4]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGVFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=281806 LL14B4_RS05360 WP_109990846.1 1084276..1085598(-) (comFA) [Lactococcus lactis subsp. lactis strain 14B4]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAACAAATTTTAGAAAGC
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTTCCTTTAGTCGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGTTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-232)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.5

90.909

0.677
  comFA/cflA Streptococcus mitis SK321

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae Rx1

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae D39

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae R6

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae TIGR4

52.284

89.545

0.468
  comFA/cflA Streptococcus mitis NCTC 12261

51.523

89.545

0.461
  comFA Bacillus subtilis subsp. subtilis str. 168

37.251

100

0.382

Multiple sequence alignment