Detailed information NTG0280103

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C4K04_RS27355
• Genome accession: NZ_CP027753
• Coordinates: 5971021..5971893 (+)
• Length: 290 a.a.
• NCBI ID: WP_124322354.1
• Uniprot ID: A0A3G7TVR5
• Organism: Pseudomonas chlororaphis strain B25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5966021..5976893

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4K04_RS27340 (C4K04_5558)	-	5968377..5968733 (-)	357	WP_009045666.1	BON domain-containing protein	-
C4K04_RS27345 (C4K04_5559)	-	5969020..5969436 (-)	417	WP_124322352.1	pilin	-
C4K04_RS27350 (C4K04_5560)	pilC	5969801..5971018 (+)	1218	WP_124322353.1	type II secretion system F family protein	Machinery gene
C4K04_RS27355 (C4K04_5561)	pilD	5971021..5971893 (+)	873	WP_124322354.1	A24 family peptidase	Machinery gene
C4K04_RS27360 (C4K04_5562)	coaE	5971890..5972513 (+)	624	WP_009045669.1	dephospho-CoA kinase	-
C4K04_RS27365 (C4K04_5563)	yacG	5972510..5972713 (+)	204	WP_009045670.1	DNA gyrase inhibitor YacG	-
C4K04_RS27370 (C4K04_5564)	-	5972718..5972933 (-)	216	WP_092420809.1	hypothetical protein	-
C4K04_RS27375 (C4K04_5565)	-	5972999..5973688 (-)	690	WP_124322355.1	energy-coupling factor ABC transporter permease	-
C4K04_RS27380 (C4K04_5566)	-	5973685..5974152 (-)	468	WP_124322356.1	FAD/FMN-containing dehydrogenase	-
C4K04_RS27385 (C4K04_5567)	-	5974606..5976042 (+)	1437	WP_124322357.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31986.13 Da
• Isoelectric Point: 7.9950

>NTDB_id=280103 C4K04_RS27355 WP_124322354.1 5971021..5971893(+) (pilD) [Pseudomonas chlororaphis strain B25]
MSLTEVLASYPLAFVASVLILGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPDEPAGPTYNLLLPHSQCPHCAHPIR
PWENIPLLSYLLLRGRCSACKAPIGRRYPLVELTCGLASAFVAWHFGFGWQAGLMLLLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGIAMLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=280103 C4K04_RS27355 WP_124322354.1 5971021..5971893(+) (pilD) [Pseudomonas chlororaphis strain B25]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGCCTTCGTAGCATCGGTGCTGATCCTGGGCCTGATTGTCGGCAG
CTTTCTCAATGTGCTGATCTGGCGGCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCCCGTGACATCCTCGGGC
TGCCCGACGAGCCTGCCGGGCCGACCTACAACCTGTTGCTCCCGCACTCCCAGTGCCCGCATTGCGCGCACCCGATCCGC
CCCTGGGAAAACATCCCGCTGTTGAGCTACCTGCTGCTGCGCGGACGCTGCTCCGCATGCAAGGCCCCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGTGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGGCT
TGATGCTGCTCCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAACTGTTGCCCGACGTGCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCACTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGCGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCCCTGGCCGGGATCGCGATGCTGCGCCGCAACCGCGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A3G7TVR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.497	98.621	0.528
pilD	Vibrio campbellii strain DS40M4	51.579	98.276	0.507
pilD	Acinetobacter baumannii D1279779	49.818	94.828	0.472
pilD	Acinetobacter nosocomialis M2	49.818	94.828	0.472
pilD	Neisseria gonorrhoeae MS11	47.794	93.793	0.448