Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   C5Z26_RS10480 Genome accession   NZ_CP027194
Coordinates   2158821..2160173 (+) Length   450 a.a.
NCBI ID   WP_105449894.1    Uniprot ID   -
Organism   Lactobacillus sp. CBA3606     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2125600..2173200 2158821..2160173 within 0


Gene organization within MGE regions


Location: 2125600..2173200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5Z26_RS10300 (C5Z26_10300) nrdF 2125600..2126616 (-) 1017 WP_105449862.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  C5Z26_RS10305 (C5Z26_10305) nrdE 2126646..2128811 (-) 2166 WP_105449863.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  C5Z26_RS10310 (C5Z26_10310) - 2128947..2129177 (-) 231 WP_105449864.1 redoxin NrdH -
  C5Z26_RS10315 (C5Z26_10315) - 2129400..2130020 (+) 621 WP_105449865.1 class I SAM-dependent methyltransferase -
  C5Z26_RS10320 (C5Z26_10320) tadA 2130013..2130516 (+) 504 WP_255414949.1 tRNA adenosine(34) deaminase TadA -
  C5Z26_RS10330 (C5Z26_10330) dnaX 2130757..2132457 (+) 1701 WP_105449866.1 DNA polymerase III subunit gamma/tau -
  C5Z26_RS10335 (C5Z26_10335) - 2132481..2132789 (+) 309 WP_105449867.1 YbaB/EbfC family nucleoid-associated protein -
  C5Z26_RS10340 (C5Z26_10340) recR 2132816..2133415 (+) 600 WP_105449868.1 recombination mediator RecR -
  C5Z26_RS10345 (C5Z26_10345) - 2133431..2133682 (+) 252 WP_105450177.1 YaaL family protein -
  C5Z26_RS10350 (C5Z26_10350) tmk 2133872..2134537 (+) 666 WP_105450178.1 dTMP kinase -
  C5Z26_RS10355 (C5Z26_10355) - 2134534..2134863 (+) 330 WP_105449869.1 cyclic-di-AMP receptor -
  C5Z26_RS10360 (C5Z26_10360) holB 2134882..2135904 (+) 1023 WP_105449870.1 DNA polymerase III subunit delta' -
  C5Z26_RS10365 (C5Z26_10365) - 2135929..2136276 (+) 348 WP_105449871.1 DNA replication initiation control protein YabA -
  C5Z26_RS10370 (C5Z26_10370) rsmI 2136299..2137177 (+) 879 WP_105449872.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  C5Z26_RS10375 (C5Z26_10375) - 2137190..2137975 (+) 786 WP_105449873.1 acyl-[acyl-carrier-protein] thioesterase -
  C5Z26_RS10380 (C5Z26_10380) galE 2138098..2139093 (+) 996 WP_105449874.1 UDP-glucose 4-epimerase GalE -
  C5Z26_RS10385 (C5Z26_10385) phnW 2139188..2140288 (-) 1101 WP_105449875.1 2-aminoethylphosphonate--pyruvate transaminase -
  C5Z26_RS10390 (C5Z26_10390) phnX 2140308..2141105 (-) 798 WP_105449876.1 phosphonoacetaldehyde hydrolase -
  C5Z26_RS10395 (C5Z26_10395) - 2141131..2141985 (-) 855 WP_105449877.1 ABC transporter permease -
  C5Z26_RS10400 (C5Z26_10400) phnE 2141982..2142785 (-) 804 WP_105449878.1 phosphonate ABC transporter, permease protein PhnE -
  C5Z26_RS10405 (C5Z26_10405) phnC 2142775..2143548 (-) 774 WP_105449879.1 phosphonate ABC transporter ATP-binding protein -
  C5Z26_RS10410 (C5Z26_10410) - 2143588..2144634 (-) 1047 WP_105449880.1 phosphate/phosphite/phosphonate ABC transporter substrate-binding protein -
  C5Z26_RS10415 (C5Z26_10415) tsaB 2144929..2145654 (+) 726 WP_105449881.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  C5Z26_RS10420 (C5Z26_10420) rimI 2145638..2146219 (+) 582 WP_105449882.1 ribosomal protein S18-alanine N-acetyltransferase -
  C5Z26_RS10425 (C5Z26_10425) rimI 2146212..2146673 (+) 462 WP_105449883.1 ribosomal protein S18-alanine N-acetyltransferase -
  C5Z26_RS10430 (C5Z26_10430) tsaD 2146686..2147723 (+) 1038 WP_105449884.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  C5Z26_RS10435 (C5Z26_10435) - 2147798..2149780 (-) 1983 WP_105449885.1 ABC-F family ATP-binding cassette domain-containing protein -
  C5Z26_RS10440 (C5Z26_10440) - 2149947..2150603 (+) 657 WP_105449886.1 redox-sensing transcriptional repressor Rex -
  C5Z26_RS10445 (C5Z26_10445) - 2150656..2151291 (-) 636 WP_105449887.1 CPBP family intramembrane glutamic endopeptidase -
  C5Z26_RS10450 (C5Z26_10450) groES 2151482..2151766 (+) 285 WP_105449888.1 co-chaperone GroES -
  C5Z26_RS10455 (C5Z26_10455) groL 2151822..2153447 (+) 1626 WP_105449889.1 chaperonin GroEL -
  C5Z26_RS10460 (C5Z26_10460) - 2153683..2155518 (+) 1836 WP_105449890.1 APC family permease -
  C5Z26_RS10465 (C5Z26_10465) - 2155682..2156779 (+) 1098 WP_105449891.1 glycosyltransferase family 4 protein -
  C5Z26_RS10470 (C5Z26_10470) - 2157094..2157993 (+) 900 WP_105449892.1 phosphate ABC transporter substrate-binding protein -
  C5Z26_RS10475 (C5Z26_10475) - 2158112..2158753 (-) 642 WP_105449893.1 YigZ family protein -
  C5Z26_RS10480 (C5Z26_10480) comFA 2158821..2160173 (+) 1353 WP_105449894.1 DEAD/DEAH box helicase Machinery gene
  C5Z26_RS10485 (C5Z26_10485) - 2160136..2160810 (+) 675 WP_105449895.1 ComF family protein -
  C5Z26_RS10490 (C5Z26_10490) hpf 2160943..2161509 (+) 567 WP_105449896.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  C5Z26_RS10495 (C5Z26_10495) secA 2161704..2164067 (+) 2364 WP_105449897.1 preprotein translocase subunit SecA -
  C5Z26_RS10500 (C5Z26_10500) prfB 2164161..2165277 (+) 1117 WP_105449898.1 peptide chain release factor 2 -
  C5Z26_RS10505 (C5Z26_10505) - 2165456..2166631 (+) 1176 WP_105449899.1 PDZ domain-containing protein -
  C5Z26_RS10510 (C5Z26_10510) - 2166637..2167362 (+) 726 WP_105449900.1 response regulator transcription factor -
  C5Z26_RS10515 (C5Z26_10515) pnpS 2167355..2168749 (+) 1395 WP_105450179.1 two-component system histidine kinase PnpS -
  C5Z26_RS10520 (C5Z26_10520) - 2168918..2169793 (+) 876 WP_105449901.1 phosphate ABC transporter substrate-binding protein PstS family protein -
  C5Z26_RS10525 (C5Z26_10525) pstC 2169804..2170733 (+) 930 WP_105449902.1 phosphate ABC transporter permease subunit PstC -
  C5Z26_RS10530 (C5Z26_10530) pstA 2170723..2171607 (+) 885 WP_105449903.1 phosphate ABC transporter permease PstA -
  C5Z26_RS10535 (C5Z26_10535) pstB 2171621..2172433 (+) 813 WP_105449904.1 phosphate ABC transporter ATP-binding protein PstB -
  C5Z26_RS10540 (C5Z26_10540) pstB 2172445..2173200 (+) 756 WP_105449905.1 phosphate ABC transporter ATP-binding protein PstB -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 50757.93 Da        Isoelectric Point: 10.6541

>NTDB_id=275689 C5Z26_RS10480 WP_105449894.1 2158821..2160173(+) (comFA) [Lactobacillus sp. CBA3606]
MQANELYGRQLNLTVAQASDLPVNTQRLMSMQVTASLVQCQRCHSRLKRAWAQLPNQHYYCYQCLNLGRISTLTSLYHIP
EPNDFPNRPKLTWTGTLTPQQTACAATIQAKFATHQRHLLWAVTGAGKTEMLFPGLAWALAQGLRVAVASPRVDVCLELY
PRLQAAFASLDIALLHGRQTEPYRYCQLTVCTTHQLLRFRAAFDVLIIDEVDAFPFAANPRLTYAVTQALKSTGALLYLT
ATPSQALLRQVRQHQLSISYLPLRFHQHLLPVIKVTLRPNWWRQIQHGQLPTVLRRWLQEYAQTQRRFLLFVPRVSQLAL
VQAAVQQNIPELTGLTVHSTDPDRLAKVQQMRSATVQYLITTTILERGVTFPGIDVIVLGADDTIFSTAALVQIAGRVGR
SKERPTGLVRFICSSYARPVKQAQRQIKQVNRKGRRLLNELSTVSTAVTP

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=275689 C5Z26_RS10480 WP_105449894.1 2158821..2160173(+) (comFA) [Lactobacillus sp. CBA3606]
ATGCAAGCGAATGAGTTATATGGTCGTCAGCTTAATTTAACGGTCGCTCAGGCTAGTGACTTACCAGTTAATACGCAACG
TTTAATGAGTATGCAAGTGACGGCTAGCTTAGTCCAGTGTCAACGTTGTCATAGTCGATTAAAGCGTGCATGGGCTCAGT
TACCTAATCAACACTATTATTGTTACCAGTGTTTAAATCTAGGCCGGATTAGCACCTTAACTTCACTGTATCACATTCCG
GAACCCAATGACTTTCCTAATCGGCCTAAATTAACTTGGACGGGGACGTTAACTCCGCAACAAACTGCTTGTGCGGCAAC
TATTCAGGCTAAGTTTGCAACACACCAACGTCATTTACTTTGGGCAGTTACGGGGGCCGGCAAGACTGAAATGTTGTTTC
CAGGGCTGGCTTGGGCATTAGCGCAAGGCCTGCGCGTTGCAGTGGCTTCACCACGGGTTGATGTTTGTTTGGAACTATAT
CCGCGGTTACAAGCGGCCTTTGCTAGCTTGGATATTGCTTTACTACATGGTCGTCAAACCGAGCCTTATCGGTATTGTCA
ATTGACCGTTTGTACCACGCATCAATTATTGCGATTTCGAGCGGCTTTTGATGTGTTAATTATTGATGAGGTAGATGCGT
TTCCTTTTGCTGCTAATCCCCGGTTGACCTATGCCGTGACCCAAGCTTTAAAATCAACAGGAGCTTTATTATATCTGACG
GCCACCCCTAGTCAAGCGTTGCTACGTCAAGTCCGGCAACATCAGTTAAGTATCAGTTATTTGCCACTACGGTTTCATCA
GCATTTATTACCGGTCATTAAGGTCACCTTACGACCAAATTGGTGGCGCCAAATTCAACACGGCCAGTTACCAACGGTAT
TACGACGCTGGTTACAGGAATATGCGCAGACGCAGCGTCGATTTTTATTGTTTGTGCCGCGAGTCAGTCAATTGGCATTA
GTCCAAGCGGCCGTGCAACAAAATATTCCGGAGTTGACCGGTTTAACGGTGCATTCTACTGATCCTGACCGGTTGGCGAA
AGTTCAGCAGATGCGTTCGGCGACGGTACAATATTTAATTACGACGACGATTTTGGAACGTGGAGTGACGTTTCCTGGTA
TTGATGTGATTGTGTTAGGCGCCGATGATACGATTTTTTCAACGGCAGCGTTAGTTCAGATTGCGGGGCGTGTCGGTCGG
TCAAAAGAGCGGCCGACTGGCTTAGTGCGTTTTATTTGTAGCAGCTATGCACGACCGGTCAAACAAGCCCAACGCCAAAT
TAAACAAGTTAATCGGAAAGGGCGGCGCCTACTAAATGAACTGTCTACTGTGTCAACGGCCGTTACACCCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(301-401)

Type III restriction enzyme, res subunit (ResIII)

(99-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

50

97.333

0.487
  comFA/cflA Streptococcus mitis NCTC 12261

40.92

91.778

0.376
  comFA Bacillus subtilis subsp. subtilis str. 168

41.133

90.222

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

39.1

93.778

0.367
  comFA/cflA Streptococcus pneumoniae D39

39.951

90.667

0.362
  comFA/cflA Streptococcus pneumoniae R6

39.951

90.667

0.362
  comFA/cflA Streptococcus pneumoniae TIGR4

39.951

90.667

0.362
  comFA/cflA Streptococcus pneumoniae Rx1

39.951

90.667

0.362
  comFA/cflA Streptococcus mitis SK321

39.706

90.667

0.36

Multiple sequence alignment