Detailed information NTG0027389

Bioinformatically predicted

Overview

• Name: ruvA
• Type: Machinery gene
• Locus tag: USA300HOU_RS08735
• Genome accession: NC_010079
• Coordinates: 1753227..1753829 (-)
• Length: 200 a.a.
• NCBI ID: WP_000271547.1
• Uniprot ID: Q2YT88
• Organism: Staphylococcus aureus subsp. aureus USA300_TCH1516
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1748227..1758829

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
USA300HOU_RS08715 (USA300HOU_1637)	yajC	1749723..1749983 (-)	261	WP_001160682.1	preprotein translocase subunit YajC	-
USA300HOU_RS08720 (USA300HOU_1638)	tgt	1750002..1751141 (-)	1140	WP_001112045.1	tRNA guanosine(34) transglycosylase Tgt	-
USA300HOU_RS08725 (USA300HOU_1639)	queA	1751164..1752189 (-)	1026	WP_001019172.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
USA300HOU_RS08730 (USA300HOU_1640)	ruvB	1752191..1753195 (-)	1005	WP_001005767.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
USA300HOU_RS08735 (USA300HOU_1641)	ruvA	1753227..1753829 (-)	603	WP_000271547.1	Holliday junction branch migration protein RuvA	Machinery gene
USA300HOU_RS08740 (USA300HOU_1642)	-	1753843..1754298 (-)	456	WP_000368902.1	ACT domain-containing protein	-
USA300HOU_RS08745 (USA300HOU_1643)	obgE	1754311..1755603 (-)	1293	WP_000496100.1	GTPase ObgE	-
USA300HOU_RS08750 (USA300HOU_1644)	rpmA	1755958..1756242 (-)	285	WP_000916187.1	50S ribosomal protein L27	-
USA300HOU_RS08755 (USA300HOU_1645)	-	1756254..1756574 (-)	321	WP_000633692.1	ribosomal-processing cysteine protease Prp	-
USA300HOU_RS08760 (USA300HOU_1646)	rplU	1756580..1756888 (-)	309	WP_000457386.1	50S ribosomal protein L21	-
USA300HOU_RS08765 (USA300HOU_1647)	mreD	1757055..1757585 (-)	531	WP_001259925.1	rod shape-determining protein MreD	-
USA300HOU_RS08770 (USA300HOU_1648)	mreC	1757585..1758427 (-)	843	WP_000911041.1	rod shape-determining protein MreC	-
USA300HOU_RS08775 (USA300HOU_1649)	-	1758627..1758746 (-)	120	WP_000840602.1	hypothetical protein	-

Sequence

Protein

• Length: 200 a.a.
• Molecular weight: 22262.46 Da
• Isoelectric Point: 6.0240

>NTDB_id=27389 USA300HOU_RS08735 WP_000271547.1 1753227..1753829(-) (ruvA) [Staphylococcus aureus subsp. aureus USA300_TCH1516]
MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEVLIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGI
GPKSALAILATSTPNEVKRAIENENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS

Nucleotide

• Length: 603 bp

>NTDB_id=27389 USA300HOU_RS08735 WP_000271547.1 1753227..1753829(-) (ruvA) [Staphylococcus aureus subsp. aureus USA300_TCH1516]
ATGTACGCGTATGTCAAAGGTAAGTTAACACATTTATATCCTACACACGTAGTTGTTGAAACTGCTGGTGTTGGTTATGA
AATTCAAACACCAAATTCTTATCGTTTTCAAAAGCATCTAGATCATGAAGTTTTAATTCATACATCTTTAATTGTTCGTG
AAGATGCACAATTATTGTATGGATTTAGTAGTGAAGAAGAGAAAGATATGTTCTTGAGTTTAATTAAAGTTACTGGTATT
GGTCCGAAATCAGCTTTAGCTATTTTAGCGACAAGTACGCCTAATGAAGTAAAACGTGCCATTGAAAATGAAAATGATAC
GTATTTAACTAAATTCCCAGGAATTGGTAAGAAAACGGCAAGACAGATTGTCTTAGATTTAAAAGGTAAAGTGAAAATTA
CTGAAGAAGATAGCGATTCATTATTACAAGTAGACGCTACTTCGACGGTGCAAGATCAATTCGTGCAAGAAGCAATGTTA
GCGTTAGAAGCATTAGGTTATTCTAAACGAGAGCTTGCAAAAGTTGAGAAAACGTTAAATAAAAATAAATATGACTCAGT
TGATGAAGCTGTTAAGGCAGGTCTTCAATTAGTTGTATCTTAA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix domain (HHH_5) (71-128)

• RuvA, C-terminal domain (RuvA_C) (156-197)

• RuvA N terminal domain (RuvA_N) (1-61)

3D structure

• AlphaFold DB: Q2YT88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvA	Streptococcus pneumoniae TIGR4	46.231	99.5	0.46
ruvA	Streptococcus pneumoniae R6	45.729	99.5	0.455
ruvA	Streptococcus pneumoniae D39	45.729	99.5	0.455
ruvA	Bacillus subtilis subsp. subtilis str. 168	42.714	99.5	0.425