Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   ECE24377A_RS19075 Genome accession   NC_009801
Coordinates   3500863..3501510 (+) Length   215 a.a.
NCBI ID   WP_001221505.1    Uniprot ID   A7ZRQ8
Organism   Escherichia coli O139:H28 str. E24377A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3495863..3506510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECE24377A_RS19050 (EcE24377A_3486) plsC 3496010..3496747 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  ECE24377A_RS19055 (EcE24377A_3487) parC 3496980..3499238 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  ECE24377A_RS19065 (EcE24377A_3488) ygiV 3499784..3500266 (-) 483 WP_000183481.1 GyrI-like domain-containing protein -
  ECE24377A_RS19070 (EcE24377A_3489) ygiW 3500319..3500711 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  ECE24377A_RS19075 (EcE24377A_3490) ciaR 3500863..3501510 (+) 648 WP_001221505.1 quorum sensing response regulator transcription factor QseB Regulator
  ECE24377A_RS19080 (EcE24377A_3491) qseC 3501507..3502856 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  ECE24377A_RS19085 ygiZ 3502902..3503234 (-) 333 WP_000912120.1 DUF2645 family protein -
  ECE24377A_RS19090 (EcE24377A_3492) mdaB 3503553..3504134 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  ECE24377A_RS19095 (EcE24377A_3493) ygiN 3504165..3504479 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  ECE24377A_RS19100 (EcE24377A_3494) parE 3504527..3506419 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 24276.12 Da        Isoelectric Point: 6.6534

>NTDB_id=27247 ECE24377A_RS19075 WP_001221505.1 3500863..3501510(+) (ciaR) [Escherichia coli O139:H28 str. E24377A]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIGLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLELLMRN
AGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 648 bp        

>NTDB_id=27247 ECE24377A_RS19075 WP_001221505.1 3500863..3501510(+) (ciaR) [Escherichia coli O139:H28 str. E24377A]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGG
GCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGCTCGACCCCGGCA
AACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTACTGATGCGTAAC
GCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAGTAATGCCGTTGA
AGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCTACACATTAGGTG
AGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-107)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(141-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZRQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.445

100

0.395
  ciaR Streptococcus pneumoniae D39

37.445

100

0.395
  ciaR Streptococcus pneumoniae R6

37.445

100

0.395
  ciaR Streptococcus pneumoniae TIGR4

37.445

100

0.395
  ciaR Streptococcus mutans UA159

34.978

100

0.363

Multiple sequence alignment