Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AM457_RS02745 Genome accession   NZ_CP026761
Coordinates   587157..587897 (-) Length   246 a.a.
NCBI ID   WP_000076102.1    Uniprot ID   A0A1E3MBI9
Organism   Acinetobacter baumannii strain AR_0078     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582157..592897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM457_RS02725 (AM457_02725) aroB 582700..583782 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  AM457_RS02730 (AM457_02730) aroK 583811..584380 (-) 570 WP_000643712.1 shikimate kinase AroK -
  AM457_RS02735 (AM457_02735) pilQ 584392..586557 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  AM457_RS02740 (AM457_02740) pilP 586619..587146 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  AM457_RS02745 (AM457_02745) pilO 587157..587897 (-) 741 WP_000076102.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM457_RS02750 (AM457_02750) pilN 587894..588535 (-) 642 WP_000201223.1 PilN domain-containing protein Machinery gene
  AM457_RS02755 (AM457_02755) pilM 588535..589593 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  AM457_RS02760 (AM457_02760) ponA 589755..592310 (+) 2556 WP_000736681.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27849.61 Da        Isoelectric Point: 4.8357

>NTDB_id=272272 AM457_RS02745 WP_000076102.1 587157..587897(-) (pilO) [Acinetobacter baumannii strain AR_0078]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGTTDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=272272 AM457_RS02745 WP_000076102.1 587157..587897(-) (pilO) [Acinetobacter baumannii strain AR_0078]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
TATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGACAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCCTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3MBI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment