Detailed information NTG0270130

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C3438_RS01930
• Genome accession: NZ_CP026533
• Coordinates: 377077..377691 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain DKU_NT_04
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 372077..382691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C3438_RS01905 (C3438_01905)	rpsT	372380..372646 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
C3438_RS01910 (C3438_01910)	holA	372663..373704 (-)	1042	Protein_380	DNA polymerase III subunit delta	-
C3438_RS22390	-	373744..373895 (-)	152	Protein_381	hypothetical protein	-
C3438_RS01915 (C3438_01915)	-	373936..374070 (+)	135	WP_003152870.1	YqzM family protein	-
C3438_RS01920 (C3438_01920)	comEC	374089..376440 (-)	2352	WP_104842690.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C3438_RS01925 (C3438_01925)	-	376441..377010 (-)	570	WP_003152868.1	ComE operon protein 2	-
C3438_RS01930 (C3438_01930)	comEA	377077..377691 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
C3438_RS01935 (C3438_01935)	comER	377750..378571 (+)	822	WP_014305449.1	late competence protein ComER	-
C3438_RS01940 (C3438_01940)	-	378640..379377 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
C3438_RS01945 (C3438_01945)	rsfS	379374..379730 (-)	357	WP_007408260.1	ribosome silencing factor	-
C3438_RS01950 (C3438_01950)	yqeK	379748..380308 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C3438_RS01955 (C3438_01955)	-	380298..380867 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
C3438_RS01960 (C3438_01960)	yhbY	380878..381168 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
C3438_RS01965 (C3438_01965)	aroE	381162..382004 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=270130 C3438_RS01930 WP_013352942.1 377077..377691(-) (comEA) [Bacillus velezensis strain DKU_NT_04]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=270130 C3438_RS01930 WP_013352942.1 377077..377691(-) (comEA) [Bacillus velezensis strain DKU_NT_04]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363