Detailed information NTG0270061

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: C3K08_RS12520
• Genome accession: NZ_CP026516
• Coordinates: 2495157..2497826 (-)
• Length: 889 a.a.
• NCBI ID: WP_104991598.1
• Uniprot ID: -
• Organism: Deinococcus sp. NW-56
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2490157..2502826

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C3K08_RS12500	-	2490661..2491824 (-)	1164	WP_104991594.1	nitric oxide synthase oxygenase	-
C3K08_RS12505	-	2491996..2492421 (-)	426	WP_104991595.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
C3K08_RS12510	-	2492488..2493867 (+)	1380	WP_104991596.1	DUF4403 family protein	-
C3K08_RS12515	pilT	2493878..2495110 (-)	1233	WP_104991597.1	type IV pilus twitching motility protein PilT	Machinery gene
C3K08_RS12520	pilB	2495157..2497826 (-)	2670	WP_104991598.1	type II/IV secretion system protein	Machinery gene
C3K08_RS12525	-	2497856..2498356 (-)	501	WP_104991599.1	YqeG family HAD IIIA-type phosphatase	-
C3K08_RS12530	pgeF	2498353..2499120 (-)	768	WP_104991600.1	peptidoglycan editing factor PgeF	-
C3K08_RS17960	-	2499169..2499354 (-)	186	WP_158679927.1	hypothetical protein	-
C3K08_RS12540	-	2499712..2500497 (+)	786	WP_104991602.1	enoyl-ACP reductase	-
C3K08_RS12545	-	2500574..2501452 (+)	879	WP_199776934.1	acyltransferase	-
C3K08_RS12550	-	2501462..2502043 (+)	582	WP_234009080.1	5-formyltetrahydrofolate cyclo-ligase	-
C3K08_RS12555	-	2502048..2502632 (+)	585	WP_104991603.1	DinB family protein	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 96080.84 Da
• Isoelectric Point: 4.8788

>NTDB_id=270061 C3K08_RS12520 WP_104991598.1 2495157..2497826(-) (pilB) [Deinococcus sp. NW-56]
MALSIGDRRLGAILLEQGYVSDTELQKALVRHAEVGGRLADILIDSGQVGEKRIARAIEEALGIPLVNLLVVTPEARAVS
AVRAQTAQAMAAFPFAREGDTLRVAFVDPLSSLAIEALEDDSGLNIEPYQALRDQVLWAIGTYYPELGLDVPLPGEEGGD
ANGGQLGRLLTTRGLITDAQLQVALDAQQQTGESLGATLVTQGALTEERLYEVLAEQAGAPYLPDTRGLQPPEDVLGALL
RADALRLSAVPVEETAAAVTVLTSDPRRREELEALIGRPVSLILARPRDVEALIERCYPQRTRLGEQMVQQGTLSRAQLR
EALQVQARGGKVKPLGEVIVELGFANPGDIDTALQKQNAGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEYLDPGQNPPD
AAVALLIPEATARRYGVVPVRLQGESLIVAMKDPRNVFALDDLKLMTGREIVPAVMAEKDITRLLERYHGNKDMAALNQK
LAAESKAREAKREDVDLSAGLDDNAVVRVVDQIIREAALQDASDIHIEPTQTALRVRYRIDGVLREQPELPRGSAQSVLA
RIKIMGNLDISERRLPQDGRVRFKKGSIDIDLRLSTLPTVYGEKAVMRLLQKAENIPEVEQLGFSAHNFQRYLDVIEKPN
GIFLVTGPTGSGKSFTSFSTLKRIARPEKNTTTIEDPIEYEIPGIVQSQVNSVAGMTFARALRAFLRQDPDIIFVGEIRD
TETAKIATEAALTGHLVLATLHTNDAPGAITRLDEMGVEPFNISASVIGVLAQRLVRRVCSECKQPTNADPDVLRRLGLA
EEEVRGASLVRGAGCPRCGGTGYKGRMGIHELMVIDDPLRRAIGAGQTASELRDVALAESGMKTLRQDGIDKALAGMTTL
EEVLAVTAG

Nucleotide

• Length: 2670 bp

>NTDB_id=270061 C3K08_RS12520 WP_104991598.1 2495157..2497826(-) (pilB) [Deinococcus sp. NW-56]
GTGGCACTTTCCATCGGCGACCGGAGACTCGGAGCGATCCTGCTCGAACAGGGCTACGTCAGTGACACCGAACTGCAAAA
GGCGCTCGTCCGGCACGCCGAGGTCGGCGGGCGGCTGGCCGACATATTGATCGACTCCGGGCAGGTCGGCGAGAAGCGCA
TCGCGCGGGCCATCGAGGAAGCGCTGGGCATTCCCCTCGTCAACCTGCTGGTGGTCACGCCGGAGGCCCGGGCGGTGTCG
GCGGTGCGGGCGCAGACGGCGCAGGCGATGGCGGCCTTTCCCTTCGCGCGGGAGGGCGACACGCTGCGGGTGGCCTTTGT
GGATCCCCTGTCCAGCCTTGCCATCGAGGCGCTGGAGGACGACTCGGGCCTGAACATCGAGCCGTATCAGGCGCTGCGCG
ATCAGGTGCTGTGGGCCATCGGGACCTACTACCCCGAACTCGGGCTGGACGTGCCCCTGCCCGGCGAGGAGGGCGGCGAC
GCGAACGGCGGGCAACTCGGCCGGTTGCTCACCACACGGGGGCTGATCACCGACGCGCAACTTCAGGTGGCACTCGATGC
CCAGCAGCAGACCGGCGAGTCGCTGGGGGCGACGCTGGTGACGCAGGGGGCGCTGACCGAGGAGCGGCTGTACGAGGTGC
TGGCCGAGCAGGCGGGCGCCCCCTACCTCCCCGACACGCGCGGCCTGCAGCCCCCGGAAGACGTGCTGGGAGCGCTGCTG
CGGGCAGACGCGCTGCGGCTCTCGGCGGTCCCGGTCGAGGAGACGGCCGCCGCCGTGACGGTCCTGACGAGCGATCCCCG
CCGCCGCGAGGAACTCGAAGCCCTGATCGGTCGCCCGGTCAGCCTGATCCTGGCCCGCCCGCGTGATGTCGAGGCCCTGA
TCGAGCGCTGCTATCCCCAGCGCACCCGCCTGGGCGAGCAGATGGTGCAGCAGGGGACGCTCTCGCGGGCACAACTGCGC
GAGGCCCTACAGGTGCAGGCGCGGGGCGGCAAGGTCAAACCACTGGGTGAGGTGATCGTCGAACTGGGCTTCGCCAACCC
CGGCGACATTGACACGGCGCTGCAAAAGCAGAACGCGGGGGGCGGGCGGCTGGAAGACACGCTGGTGCAGTCGGGCAAGC
TCAGCCCCGAGATGCTGGCCCGCTCCTTGGCCGCGCAGCTCGGCTACGAGTACCTCGACCCCGGCCAGAACCCGCCCGAC
GCGGCGGTGGCCCTCCTGATCCCGGAGGCGACCGCCCGGCGCTACGGGGTGGTGCCCGTGCGGCTGCAGGGCGAGTCGCT
GATCGTGGCGATGAAGGACCCGCGCAACGTCTTCGCGCTCGACGACCTTAAGCTGATGACGGGCCGCGAGATCGTTCCCG
CCGTGATGGCGGAAAAGGACATCACCCGGTTGCTGGAGCGCTACCACGGCAACAAGGACATGGCGGCCCTGAACCAGAAG
CTCGCCGCCGAGAGCAAGGCGCGGGAGGCCAAGCGCGAGGACGTGGACCTCTCCGCCGGACTCGACGACAACGCCGTCGT
GCGGGTGGTGGACCAGATCATCCGGGAGGCGGCCCTGCAGGACGCCTCGGACATCCACATCGAGCCGACCCAGACGGCCC
TGCGGGTCCGCTACCGCATCGACGGCGTGCTGCGCGAACAGCCCGAGTTGCCGCGCGGCAGCGCCCAGAGCGTGCTCGCC
CGCATCAAGATCATGGGGAACCTCGACATCTCCGAGCGCCGCCTGCCGCAAGACGGCCGCGTGCGCTTCAAGAAGGGCTC
GATCGACATCGACCTCCGGCTCTCGACCCTGCCCACCGTGTACGGGGAAAAGGCCGTGATGCGCCTCCTGCAAAAGGCCG
AGAACATCCCCGAGGTCGAGCAGCTCGGCTTTTCCGCGCACAACTTCCAGCGTTACCTCGACGTGATCGAAAAGCCCAAC
GGCATCTTCCTGGTGACGGGGCCGACGGGGTCGGGCAAGTCCTTTACCAGTTTCTCGACCCTCAAGCGCATCGCCCGGCC
CGAGAAGAACACCACGACCATCGAGGACCCCATCGAGTACGAGATTCCGGGGATCGTGCAGTCGCAGGTCAACTCGGTGG
CGGGGATGACCTTTGCCCGTGCCCTCCGCGCCTTCCTGCGTCAGGACCCCGACATCATCTTCGTGGGCGAAATCCGCGAC
ACCGAGACCGCCAAGATTGCCACCGAAGCGGCGCTCACCGGCCACCTCGTGCTGGCGACCCTGCACACGAACGACGCGCC
GGGAGCGATCACCCGCCTCGACGAGATGGGCGTGGAGCCGTTCAACATCTCAGCCTCGGTGATTGGGGTGCTCGCGCAGC
GGCTGGTGCGCCGGGTGTGCTCCGAGTGCAAGCAGCCCACCAACGCCGACCCCGACGTGCTGCGCCGCTTGGGGCTGGCC
GAGGAGGAGGTGCGCGGCGCCTCGCTCGTGCGCGGGGCAGGCTGCCCCCGCTGCGGCGGCACCGGCTACAAGGGCCGCAT
GGGGATTCACGAGCTGATGGTGATCGATGACCCCCTGCGCCGCGCCATCGGGGCCGGGCAGACCGCCTCCGAACTGCGCG
ACGTGGCCCTGGCCGAGAGCGGCATGAAGACGCTGCGCCAGGACGGCATCGACAAGGCGCTCGCGGGGATGACCACCCTG
GAAGAAGTGCTCGCAGTGACGGCGGGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-778)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	75.928	100	0.759
pilF	Thermus thermophilus HB27	57.175	100	0.574