Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C2U39_RS13490 Genome accession   NZ_CP026222
Coordinates   2858633..2859769 (+) Length   378 a.a.
NCBI ID   WP_029314740.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2853633..2864769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U39_RS13460 (C2U39_13460) - 2853735..2854115 (-) 381 WP_041213103.1 diacylglycerol kinase -
  C2U39_RS13465 (C2U39_13465) - 2854257..2855321 (+) 1065 WP_103260349.1 3-deoxy-7-phosphoheptulonate synthase -
  C2U39_RS13470 (C2U39_13470) - 2855533..2856306 (-) 774 WP_103260350.1 ATP-binding cassette domain-containing protein -
  C2U39_RS13475 (C2U39_13475) btuC 2856293..2857300 (-) 1008 WP_103260351.1 vitamin B12 ABC transporter permease BtuC -
  C2U39_RS13480 (C2U39_13480) - 2857376..2858107 (-) 732 WP_039041212.1 transporter substrate-binding domain-containing protein -
  C2U39_RS13485 (C2U39_13485) - 2858220..2858576 (+) 357 WP_010675200.1 DMT family protein -
  C2U39_RS13490 (C2U39_13490) pilU 2858633..2859769 (+) 1137 WP_029314740.1 type IVa pilus ATPase TapW Machinery gene
  C2U39_RS13495 (C2U39_13495) - 2859830..2860072 (-) 243 WP_010675202.1 FeoC-like transcriptional regulator -
  C2U39_RS13500 (C2U39_13500) feoB 2860119..2862389 (-) 2271 WP_103260352.1 Fe(2+) transporter permease subunit FeoB -
  C2U39_RS13505 (C2U39_13505) - 2862386..2862613 (-) 228 WP_010675204.1 FeoA family protein -
  C2U39_RS13510 (C2U39_13510) - 2862733..2863062 (+) 330 WP_042864056.1 translation initiation factor -
  C2U39_RS13515 (C2U39_13515) - 2863316..2863873 (+) 558 WP_103260353.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42203.27 Da        Isoelectric Point: 6.6000

>NTDB_id=267893 C2U39_RS13490 WP_029314740.1 2858633..2859769(+) (pilU) [Aeromonas sp. ASNIH3]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=267893 C2U39_RS13490 WP_029314740.1 2858633..2859769(+) (pilU) [Aeromonas sp. ASNIH3]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACCCAGGAGCCTCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCCCCGGTACTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAGTCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGTGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGTTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTGATGCTCGGAACCCACACGGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGCACTCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment