Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   C0971_RS12270 Genome accession   NZ_CP026143
Coordinates   2417215..2418297 (-) Length   360 a.a.
NCBI ID   WP_248891851.1    Uniprot ID   -
Organism   Bacillus methanolicus strain DFS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2412215..2423297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS12225 (C0971_12285) - 2412752..2413543 (+) 792 WP_004435620.1 YqhG family protein -
  C0971_RS12230 (C0971_12290) - 2413589..2413765 (-) 177 WP_081485685.1 YqzE family protein -
  C0971_RS12235 (C0971_12295) - 2413808..2414311 (-) 504 WP_248891845.1 shikimate kinase -
  C0971_RS12240 (C0971_12300) comGG 2414325..2414711 (-) 387 WP_004435627.1 competence type IV pilus minor pilin ComGG -
  C0971_RS12245 (C0971_12305) comGF 2414708..2415181 (-) 474 WP_248891847.1 competence type IV pilus minor pilin ComGF -
  C0971_RS12250 (C0971_12310) - 2415132..2415461 (-) 330 WP_248891848.1 hypothetical protein -
  C0971_RS12255 (C0971_12315) comGD 2415436..2415885 (-) 450 WP_004435633.1 competence type IV pilus minor pilin ComGD -
  C0971_RS12260 (C0971_12320) comGC 2415882..2416193 (-) 312 WP_004435635.1 competence type IV pilus major pilin ComGC Machinery gene
  C0971_RS12265 (C0971_12325) comGB 2416209..2417240 (-) 1032 WP_248891850.1 competence type IV pilus assembly protein ComGB -
  C0971_RS12270 (C0971_12330) comGA 2417215..2418297 (-) 1083 WP_248891851.1 competence type IV pilus ATPase ComGA Machinery gene
  C0971_RS12275 (C0971_12340) - 2418657..2419358 (+) 702 WP_248891853.1 helix-turn-helix domain-containing protein -
  C0971_RS12280 (C0971_12345) - 2419442..2419687 (+) 246 WP_004435645.1 DUF2626 domain-containing protein -
  C0971_RS12285 (C0971_12350) - 2419725..2420834 (-) 1110 WP_248891854.1 SAM-dependent methyltransferase -
  C0971_RS12290 (C0971_12355) - 2421023..2421658 (-) 636 WP_004435650.1 MBL fold metallo-hydrolase -
  C0971_RS12295 (C0971_12360) - 2421737..2422231 (+) 495 WP_248891856.1 hypothetical protein -
  C0971_RS12300 (C0971_12365) - 2422328..2422501 (+) 174 WP_004435655.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40622.96 Da        Isoelectric Point: 8.2367

>NTDB_id=267524 C0971_RS12270 WP_248891851.1 2417215..2418297(-) (comGA) [Bacillus methanolicus strain DFS2]
MSSIKQLADRIIRDAVRNQATDIHIIPRKKDTLIQLRMGNRLVPRLYLPKEECDRLISHFKFTALMDIGERRRPQSGAFS
FIVDGDAIGLRLSTLPANSSESLVIRILPQQKQIPFYQLSLFPSITRKLLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHLFNRNVITLEDPIEKDADSVLQVQVNEKAGVSYAAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAKGAIYRLIEFGVNWLEVEQTLVAVTAQRLVELTCPFCEEECSPYCFSYGRWKRASVFEILHGRALSSVMRRANGED
CEASYRTLKEMIKKGIALGYIKESEYDRWVYDNESLEMEG

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=267524 C0971_RS12270 WP_248891851.1 2417215..2418297(-) (comGA) [Bacillus methanolicus strain DFS2]
GTGAGTTCAATAAAACAGTTGGCTGACAGAATTATAAGAGATGCTGTCCGAAATCAAGCTACAGACATTCACATTATCCC
GAGAAAAAAAGATACCCTGATACAGCTTCGAATGGGCAACCGGCTAGTCCCTCGACTTTACCTCCCAAAAGAAGAATGTG
ACCGACTCATATCTCATTTTAAATTTACGGCTTTAATGGATATCGGAGAAAGAAGGCGTCCCCAGAGCGGAGCTTTTTCC
TTCATAGTCGATGGCGATGCAATCGGACTTCGTTTATCCACCCTTCCTGCCAACAGTAGTGAAAGTCTTGTTATCCGAAT
ACTTCCTCAGCAAAAGCAAATTCCTTTTTATCAATTATCCTTATTTCCAAGCATTACACGAAAACTATTAGCGCTACTTA
AACACGCTCATGGCCTAATTATTTTCACCGGACCAACCGGAAGCGGCAAGACCACTACCCTTTATTCACTCCTAAATGAA
ACTTCCCATTTATTTAATCGAAATGTGATTACGTTAGAGGATCCGATCGAAAAAGATGCTGATTCTGTTCTGCAAGTGCA
AGTAAATGAAAAAGCGGGAGTATCGTATGCTGCTGGCTTAAAAGCTATTTTGCGGCACGACCCTGACATTATCATGGTCG
GAGAGATAAGAGATGCGGAAACAGCAAAAATTGCCGTTCGGGCTGCTTTAACGGGACATCTTGTTTTGAGCACAATGCAT
ACAAGGGATGCGAAGGGAGCGATCTACAGGCTGATAGAATTTGGTGTGAATTGGCTAGAAGTAGAACAAACGCTTGTAGC
GGTAACTGCCCAGCGTCTTGTCGAATTAACTTGTCCATTTTGCGAAGAAGAGTGCTCTCCTTATTGCTTTTCGTACGGAA
GGTGGAAAAGAGCAAGTGTTTTCGAGATTTTGCACGGCCGGGCTCTATCCTCTGTCATGAGAAGAGCTAACGGAGAAGAT
TGTGAGGCTTCGTACAGGACGTTAAAGGAAATGATCAAAAAAGGAATTGCACTTGGCTATATTAAGGAATCGGAATATGA
CAGGTGGGTGTATGATAATGAATCGCTCGAAATGGAAGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.102

97.778

0.558
  pilB Glaesserella parasuis strain SC1401

40

97.222

0.389
  pilB Haemophilus influenzae 86-028NP

36.932

97.778

0.361

Multiple sequence alignment