Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   C0971_RS12260 Genome accession   NZ_CP026143
Coordinates   2415882..2416193 (-) Length   103 a.a.
NCBI ID   WP_004435635.1    Uniprot ID   I3EAF6
Organism   Bacillus methanolicus strain DFS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2410882..2421193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS12220 (C0971_12280) - 2411086..2412783 (+) 1698 WP_248891844.1 SNF2-related protein -
  C0971_RS12225 (C0971_12285) - 2412752..2413543 (+) 792 WP_004435620.1 YqhG family protein -
  C0971_RS12230 (C0971_12290) - 2413589..2413765 (-) 177 WP_081485685.1 YqzE family protein -
  C0971_RS12235 (C0971_12295) - 2413808..2414311 (-) 504 WP_248891845.1 shikimate kinase -
  C0971_RS12240 (C0971_12300) comGG 2414325..2414711 (-) 387 WP_004435627.1 competence type IV pilus minor pilin ComGG -
  C0971_RS12245 (C0971_12305) comGF 2414708..2415181 (-) 474 WP_248891847.1 competence type IV pilus minor pilin ComGF -
  C0971_RS12250 (C0971_12310) - 2415132..2415461 (-) 330 WP_248891848.1 hypothetical protein -
  C0971_RS12255 (C0971_12315) comGD 2415436..2415885 (-) 450 WP_004435633.1 competence type IV pilus minor pilin ComGD -
  C0971_RS12260 (C0971_12320) comGC 2415882..2416193 (-) 312 WP_004435635.1 competence type IV pilus major pilin ComGC Machinery gene
  C0971_RS12265 (C0971_12325) comGB 2416209..2417240 (-) 1032 WP_248891850.1 competence type IV pilus assembly protein ComGB -
  C0971_RS12270 (C0971_12330) comGA 2417215..2418297 (-) 1083 WP_248891851.1 competence type IV pilus ATPase ComGA Machinery gene
  C0971_RS12275 (C0971_12340) - 2418657..2419358 (+) 702 WP_248891853.1 helix-turn-helix domain-containing protein -
  C0971_RS12280 (C0971_12345) - 2419442..2419687 (+) 246 WP_004435645.1 DUF2626 domain-containing protein -
  C0971_RS12285 (C0971_12350) - 2419725..2420834 (-) 1110 WP_248891854.1 SAM-dependent methyltransferase -

Sequence


Protein


Download         Length: 103 a.a.        Molecular weight: 11471.45 Da        Isoelectric Point: 7.1325

>NTDB_id=267523 C0971_RS12260 WP_004435635.1 2415882..2416193(-) (comGC) [Bacillus methanolicus strain DFS2]
MKNEKGFTLIEMLIVLLVISILLIITIPNITKHNDNINNKGCEAFIKMVQAQVQAYEIEHKVYPQSVKDLISKGYLNEKE
DKCPNGKEVIITNGVAAVKEGSS

Nucleotide


Download         Length: 312 bp        

>NTDB_id=267523 C0971_RS12260 WP_004435635.1 2415882..2416193(-) (comGC) [Bacillus methanolicus strain DFS2]
TTGAAAAATGAAAAAGGTTTTACGTTAATAGAAATGTTGATTGTTTTGCTCGTCATTTCAATTTTGTTAATAATTACGAT
TCCAAATATTACAAAGCACAATGACAATATTAACAATAAAGGCTGTGAAGCATTCATAAAAATGGTTCAGGCTCAAGTCC
AGGCATACGAAATTGAACATAAGGTTTATCCTCAATCTGTAAAAGACCTGATTTCAAAAGGTTATCTTAATGAAAAAGAA
GATAAGTGTCCAAATGGGAAAGAAGTTATAATTACGAACGGTGTCGCAGCTGTGAAAGAAGGCTCTTCATGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB I3EAF6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

57.732

94.175

0.544

  comGC Staphylococcus aureus MW2

46.875

93.204

0.437

  comGC Staphylococcus aureus N315

46.875

93.204

0.437


Multiple sequence alignment