Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C2I05_RS02685 Genome accession   NZ_CP026039
Coordinates   466501..467118 (-) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis strain PK1_2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 461501..472118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2I05_RS02655 (C2I05_02640) rpsT 461803..462069 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  C2I05_RS02660 (C2I05_02645) holA 462084..463127 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  C2I05_RS02665 - 463167..463280 (-) 114 WP_122060479.1 hypothetical protein -
  C2I05_RS02670 (C2I05_02650) yqzM 463357..463491 (+) 135 WP_003229983.1 YqzM family protein -
  C2I05_RS02675 (C2I05_02655) comEC 463532..465861 (-) 2330 Protein_533 DNA internalization-related competence protein ComEC/Rec2 -
  C2I05_RS02680 (C2I05_02660) comEB 465865..466434 (-) 570 WP_072174291.1 ComE operon protein 2 -
  C2I05_RS02685 (C2I05_02665) comEA 466501..467118 (-) 618 WP_032726193.1 competence protein ComEA Machinery gene
  C2I05_RS02690 (C2I05_02670) comER 467202..468023 (+) 822 WP_014477389.1 late competence protein ComER -
  C2I05_RS02695 (C2I05_02675) yqeM 468089..468832 (-) 744 WP_072174289.1 class I SAM-dependent methyltransferase -
  C2I05_RS02700 (C2I05_02680) rsfS 468829..469185 (-) 357 WP_014477391.1 ribosome silencing factor -
  C2I05_RS02705 (C2I05_02685) yqeK 469203..469763 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C2I05_RS02710 (C2I05_02690) nadD 469753..470322 (-) 570 WP_021480066.1 nicotinate-nucleotide adenylyltransferase -
  C2I05_RS02715 (C2I05_02695) yhbY 470334..470624 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C2I05_RS02720 (C2I05_02700) aroE 470618..471460 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=265831 C2I05_RS02685 WP_032726193.1 466501..467118(-) (comEA) [Bacillus subtilis strain PK1_2]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=265831 C2I05_RS02685 WP_032726193.1 466501..467118(-) (comEA) [Bacillus subtilis strain PK1_2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTACAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment