Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C2H97_RS00680 Genome accession   NZ_CP026038
Coordinates   115741..116358 (-) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis PY79 strain PK1_3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 110741..121358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H97_RS00650 (C2H97_00665) rpsT 111042..111308 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  C2H97_RS00655 (C2H97_00670) holA 111323..112366 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  C2H97_RS00660 - 112406..112519 (-) 114 WP_122060479.1 hypothetical protein -
  C2H97_RS00665 (C2H97_00675) yqzM 112596..112730 (+) 135 WP_003229983.1 YqzM family protein -
  C2H97_RS00670 (C2H97_00680) comEC 112771..115101 (-) 2331 WP_249848131.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C2H97_RS00675 (C2H97_00685) comEB 115105..115674 (-) 570 WP_072174291.1 ComE operon protein 2 -
  C2H97_RS00680 (C2H97_00690) comEA 115741..116358 (-) 618 WP_032726193.1 competence protein ComEA Machinery gene
  C2H97_RS00685 (C2H97_00695) comER 116442..117263 (+) 822 WP_014477389.1 late competence protein ComER -
  C2H97_RS00690 (C2H97_00700) yqeM 117329..118072 (-) 744 WP_072174289.1 class I SAM-dependent methyltransferase -
  C2H97_RS00695 (C2H97_00705) rsfS 118069..118425 (-) 357 WP_014477391.1 ribosome silencing factor -
  C2H97_RS00700 (C2H97_00710) yqeK 118443..119003 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C2H97_RS00705 (C2H97_00715) nadD 118993..119562 (-) 570 WP_021480066.1 nicotinate-nucleotide adenylyltransferase -
  C2H97_RS00710 (C2H97_00720) yhbY 119574..119864 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C2H97_RS00715 (C2H97_00725) aroE 119858..120700 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=265750 C2H97_RS00680 WP_032726193.1 115741..116358(-) (comEA) [Bacillus subtilis PY79 strain PK1_3]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=265750 C2H97_RS00680 WP_032726193.1 115741..116358(-) (comEA) [Bacillus subtilis PY79 strain PK1_3]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTACAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment