Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C2H93_RS10905 Genome accession   NZ_CP026036
Coordinates   2144675..2145292 (-) Length   205 a.a.
NCBI ID   WP_077671454.1    Uniprot ID   -
Organism   Bacillus subtilis strain PK5_16     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2139675..2150292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H93_RS10875 (C2H93_10900) rpsT 2139977..2140243 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  C2H93_RS10880 (C2H93_10905) holA 2140258..2141301 (-) 1044 WP_141949661.1 DNA polymerase III subunit delta -
  C2H93_RS10885 - 2141341..2141490 (-) 150 WP_003229985.1 hypothetical protein -
  C2H93_RS10890 (C2H93_10910) yqzM 2141531..2141665 (+) 135 WP_003229983.1 YqzM family protein -
  C2H93_RS10895 (C2H93_10915) comEC 2141705..2144035 (-) 2331 WP_249852191.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C2H93_RS10900 (C2H93_10920) comEB 2144039..2144608 (-) 570 WP_072174291.1 ComE operon protein 2 -
  C2H93_RS10905 (C2H93_10925) comEA 2144675..2145292 (-) 618 WP_077671454.1 competence protein ComEA Machinery gene
  C2H93_RS10910 (C2H93_10930) comER 2145376..2146197 (+) 822 WP_021480064.1 late competence protein ComER -
  C2H93_RS10915 (C2H93_10935) yqeM 2146263..2147006 (-) 744 WP_072174289.1 class I SAM-dependent methyltransferase -
  C2H93_RS10920 (C2H93_10940) rsfS 2147003..2147359 (-) 357 WP_014477391.1 ribosome silencing factor -
  C2H93_RS10925 (C2H93_10945) yqeK 2147377..2147937 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C2H93_RS10930 (C2H93_10950) nadD 2147927..2148496 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  C2H93_RS10935 (C2H93_10955) yhbY 2148508..2148798 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C2H93_RS10940 (C2H93_10960) aroE 2148792..2149634 (-) 843 WP_249852192.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21756.45 Da        Isoelectric Point: 4.7284

>NTDB_id=265628 C2H93_RS10905 WP_077671454.1 2144675..2145292(-) (comEA) [Bacillus subtilis strain PK5_16]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=265628 C2H93_RS10905 WP_077671454.1 2144675..2145292(-) (comEA) [Bacillus subtilis strain PK5_16]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCAAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment