Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C2H96_RS19675 Genome accession   NZ_CP026035
Coordinates   3831986..3832603 (+) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis strain PK5_68     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3826986..3837603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H96_RS19640 (C2H96_19580) aroE 3827644..3828486 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  C2H96_RS19645 (C2H96_19585) yhbY 3828480..3828770 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C2H96_RS19650 (C2H96_19590) nadD 3828782..3829351 (+) 570 WP_021480066.1 nicotinate-nucleotide adenylyltransferase -
  C2H96_RS19655 (C2H96_19595) yqeK 3829341..3829901 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C2H96_RS19660 (C2H96_19600) rsfS 3829919..3830275 (+) 357 WP_014477391.1 ribosome silencing factor -
  C2H96_RS19665 (C2H96_19605) yqeM 3830272..3831015 (+) 744 WP_072174289.1 class I SAM-dependent methyltransferase -
  C2H96_RS19670 (C2H96_19610) comER 3831081..3831902 (-) 822 WP_014477389.1 late competence protein ComER -
  C2H96_RS19675 (C2H96_19615) comEA 3831986..3832603 (+) 618 WP_032726193.1 competence protein ComEA Machinery gene
  C2H96_RS19680 (C2H96_19620) comEB 3832670..3833239 (+) 570 WP_072174291.1 ComE operon protein 2 -
  C2H96_RS19685 (C2H96_19625) comEC 3833243..3835573 (+) 2331 WP_249848131.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C2H96_RS19690 (C2H96_19630) yqzM 3835614..3835748 (-) 135 WP_003229983.1 YqzM family protein -
  C2H96_RS19695 - 3835825..3835938 (+) 114 WP_122060479.1 hypothetical protein -
  C2H96_RS19700 (C2H96_19635) holA 3835978..3837021 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  C2H96_RS19705 (C2H96_19640) rpsT 3837036..3837302 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=265566 C2H96_RS19675 WP_032726193.1 3831986..3832603(+) (comEA) [Bacillus subtilis strain PK5_68]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=265566 C2H96_RS19675 WP_032726193.1 3831986..3832603(+) (comEA) [Bacillus subtilis strain PK5_68]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTACAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment