Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C2H94_RS02190 Genome accession   NZ_CP026034
Coordinates   373997..374614 (-) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis strain PK5_52     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368997..379614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H94_RS02160 (C2H94_02150) rpsT 369298..369564 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  C2H94_RS02165 (C2H94_02155) holA 369579..370622 (-) 1044 WP_167568607.1 DNA polymerase III subunit delta -
  C2H94_RS02170 - 370662..370775 (-) 114 WP_122060479.1 hypothetical protein -
  C2H94_RS02175 (C2H94_02160) yqzM 370852..370986 (+) 135 WP_003229983.1 YqzM family protein -
  C2H94_RS02180 (C2H94_02165) comEC 371027..373357 (-) 2331 WP_167568605.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C2H94_RS02185 (C2H94_02170) comEB 373361..373930 (-) 570 WP_003229978.1 ComE operon protein 2 -
  C2H94_RS02190 (C2H94_02175) comEA 373997..374614 (-) 618 WP_032726193.1 competence protein ComEA Machinery gene
  C2H94_RS02195 (C2H94_02180) comER 374698..375519 (+) 822 WP_032726195.1 late competence protein ComER -
  C2H94_RS02200 (C2H94_02185) yqeM 375585..376328 (-) 744 WP_032726197.1 class I SAM-dependent methyltransferase -
  C2H94_RS02205 (C2H94_02190) rsfS 376325..376681 (-) 357 WP_014477391.1 ribosome silencing factor -
  C2H94_RS02210 (C2H94_02195) yqeK 376699..377259 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C2H94_RS02215 (C2H94_02200) nadD 377249..377818 (-) 570 WP_021480066.1 nicotinate-nucleotide adenylyltransferase -
  C2H94_RS02220 (C2H94_02205) yhbY 377830..378120 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C2H94_RS02225 (C2H94_02210) aroE 378114..378956 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=265440 C2H94_RS02190 WP_032726193.1 373997..374614(-) (comEA) [Bacillus subtilis strain PK5_52]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=265440 C2H94_RS02190 WP_032726193.1 373997..374614(-) (comEA) [Bacillus subtilis strain PK5_52]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment