Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C2H91_RS18040 Genome accession   NZ_CP026030
Coordinates   3603073..3603690 (+) Length   205 a.a.
NCBI ID   WP_032726193.1    Uniprot ID   -
Organism   Bacillus subtilis strain PK3_9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3598073..3608690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H91_RS18005 (C2H91_18025) aroE 3598731..3599573 (+) 843 WP_249850932.1 shikimate dehydrogenase -
  C2H91_RS18010 (C2H91_18030) yhbY 3599567..3599857 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  C2H91_RS18015 (C2H91_18035) nadD 3599869..3600438 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  C2H91_RS18020 (C2H91_18040) yqeK 3600428..3600988 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  C2H91_RS18025 (C2H91_18045) rsfS 3601006..3601362 (+) 357 WP_014477391.1 ribosome silencing factor -
  C2H91_RS18030 (C2H91_18050) yqeM 3601359..3602102 (+) 744 WP_124072952.1 class I SAM-dependent methyltransferase -
  C2H91_RS18035 (C2H91_18055) comER 3602168..3602989 (-) 822 WP_014480313.1 late competence protein ComER -
  C2H91_RS18040 (C2H91_18060) comEA 3603073..3603690 (+) 618 WP_032726193.1 competence protein ComEA Machinery gene
  C2H91_RS18045 (C2H91_18065) comEB 3603757..3604326 (+) 570 WP_003229978.1 ComE operon protein 2 -
  C2H91_RS18050 (C2H91_18070) comEC 3604330..3606660 (+) 2331 WP_163136229.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C2H91_RS18055 (C2H91_18075) yqzM 3606701..3606835 (-) 135 WP_003229983.1 YqzM family protein -
  C2H91_RS18060 - 3606912..3607025 (+) 114 WP_134975484.1 hypothetical protein -
  C2H91_RS18065 (C2H91_18080) holA 3607065..3608108 (+) 1044 WP_021480061.1 DNA polymerase III subunit delta -
  C2H91_RS18070 (C2H91_18085) rpsT 3608123..3608389 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21753.50 Da        Isoelectric Point: 4.8417

>NTDB_id=265300 C2H91_RS18040 WP_032726193.1 3603073..3603690(+) (comEA) [Bacillus subtilis strain PK3_9]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=265300 C2H91_RS18040 WP_032726193.1 3603073..3603690(+) (comEA) [Bacillus subtilis strain PK3_9]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376

Multiple sequence alignment