Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   DXE52_RS18950 Genome accession   NZ_CP031347
Coordinates   3758482..3759141 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O145 strain RM9873-C1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3753482..3764141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE52_RS18925 (DXE52_19515) ygiS 3754765..3756372 (-) 1608 WP_001400746.1 ABC transporter substrate-binding protein -
  DXE52_RS18930 (DXE52_19520) mqsA 3756505..3756900 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  DXE52_RS18935 (DXE52_19525) mqsR 3756902..3757198 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  DXE52_RS18940 (DXE52_19530) ygiV 3757403..3757885 (-) 483 WP_000183497.1 GyrI-like domain-containing protein -
  DXE52_RS18945 (DXE52_19535) ygiW 3757938..3758330 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  DXE52_RS18950 (DXE52_19540) ciaR 3758482..3759141 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  DXE52_RS18955 (DXE52_19545) qseC 3759138..3760487 (+) 1350 WP_000673396.1 quorum sensing histidine kinase QseC -
  DXE52_RS18960 (DXE52_19550) - 3760569..3760865 (-) 297 WP_000912118.1 DUF2645 family protein -
  DXE52_RS18965 (DXE52_19555) mdaB 3761223..3761804 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  DXE52_RS18970 (DXE52_19560) ygiN 3761835..3762149 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  DXE52_RS18975 (DXE52_19565) parE 3762197..3764089 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=265133 DXE52_RS18950 WP_001221502.1 3758482..3759141(+) (ciaR) [Escherichia coli O145 strain RM9873-C1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=265133 DXE52_RS18950 WP_001221502.1 3758482..3759141(+) (ciaR) [Escherichia coli O145 strain RM9873-C1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment