Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   DXE53_RS19575 Genome accession   NZ_CP031345
Coordinates   3845040..3845699 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O145 strain RM12367-C1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3840040..3850699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE53_RS19550 (DXE53_20185) ygiS 3841323..3842930 (-) 1608 WP_001400746.1 ABC transporter substrate-binding protein -
  DXE53_RS19555 (DXE53_20190) mqsA 3843063..3843458 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  DXE53_RS19560 (DXE53_20195) mqsR 3843460..3843756 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  DXE53_RS19565 (DXE53_20200) ygiV 3843961..3844443 (-) 483 WP_000183497.1 GyrI-like domain-containing protein -
  DXE53_RS19570 (DXE53_20205) ygiW 3844496..3844888 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  DXE53_RS19575 (DXE53_20210) ciaR 3845040..3845699 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  DXE53_RS19580 (DXE53_20215) qseC 3845696..3847045 (+) 1350 WP_000673396.1 quorum sensing histidine kinase QseC -
  DXE53_RS19585 (DXE53_20220) - 3847127..3847423 (-) 297 WP_000912118.1 DUF2645 family protein -
  DXE53_RS19590 (DXE53_20225) mdaB 3847781..3848362 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  DXE53_RS19595 (DXE53_20230) ygiN 3848393..3848707 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  DXE53_RS19600 (DXE53_20235) parE 3848755..3850647 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=265057 DXE53_RS19575 WP_001221502.1 3845040..3845699(+) (ciaR) [Escherichia coli O145 strain RM12367-C1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=265057 DXE53_RS19575 WP_001221502.1 3845040..3845699(+) (ciaR) [Escherichia coli O145 strain RM12367-C1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365