Detailed information NTG0265014

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BS11774_RS19085
• Genome accession: NZ_CP026010
• Coordinates: 3574250..3574867 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain ATCC 11774
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3569250..3579867

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS11774_RS19055 (BS11774_18945)	rpsT	3569552..3569818 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
BS11774_RS19060 (BS11774_18950)	holA	3569833..3570876 (-)	1044	WP_128474068.1	DNA polymerase III subunit delta	-
BS11774_RS19065	-	3570916..3571065 (-)	150	WP_128474069.1	hypothetical protein	-
BS11774_RS19070 (BS11774_18955)	yqzM	3571106..3571240 (+)	135	WP_003229983.1	YqzM family protein	-
BS11774_RS19075 (BS11774_18960)	comEC	3571280..3573610 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BS11774_RS19080 (BS11774_18965)	comEB	3573614..3574183 (-)	570	WP_003229978.1	ComE operon protein 2	-
BS11774_RS19085 (BS11774_18970)	comEA	3574250..3574867 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
BS11774_RS19090 (BS11774_18975)	comER	3574951..3575772 (+)	822	WP_128474070.1	late competence protein ComER	-
BS11774_RS19095 (BS11774_18980)	yqeM	3575838..3576581 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
BS11774_RS19100 (BS11774_18985)	rsfS	3576578..3576934 (-)	357	WP_041333759.1	ribosome silencing factor	-
BS11774_RS19105 (BS11774_18990)	yqeK	3576952..3577512 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BS11774_RS19110 (BS11774_18995)	nadD	3577502..3578071 (-)	570	WP_041333756.1	nicotinate-nucleotide adenylyltransferase	-
BS11774_RS19115 (BS11774_19000)	yhbY	3578083..3578373 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
BS11774_RS19120 (BS11774_19005)	aroE	3578367..3579209 (-)	843	WP_029317883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=265014 BS11774_RS19085 WP_004398514.1 3574250..3574867(-) (comEA) [Bacillus subtilis strain ATCC 11774]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=265014 BS11774_RS19085 WP_004398514.1 3574250..3574867(-) (comEA) [Bacillus subtilis strain ATCC 11774]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39