Detailed information NTG0264980

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: DXE54_RS18730
• Genome accession: NZ_CP031343
• Coordinates: 3715678..3716337 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli O145 strain RM10425-C1
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3710678..3721337

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DXE54_RS18705 (DXE54_19285)	ygiS	3711961..3713568 (-)	1608	WP_001400746.1	ABC transporter substrate-binding protein	-
DXE54_RS18710 (DXE54_19290)	mqsA	3713701..3714096 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
DXE54_RS18715 (DXE54_19295)	mqsR	3714098..3714394 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
DXE54_RS18720 (DXE54_19300)	ygiV	3714599..3715081 (-)	483	WP_000183497.1	GyrI-like domain-containing protein	-
DXE54_RS18725 (DXE54_19305)	ygiW	3715134..3715526 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
DXE54_RS18730 (DXE54_19310)	ciaR	3715678..3716337 (+)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
DXE54_RS18735 (DXE54_19315)	qseC	3716334..3717683 (+)	1350	WP_000673396.1	quorum sensing histidine kinase QseC	-
DXE54_RS18740 (DXE54_19320)	-	3717765..3718061 (-)	297	WP_000912118.1	DUF2645 family protein	-
DXE54_RS18745 (DXE54_19325)	mdaB	3718419..3719000 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
DXE54_RS18750 (DXE54_19330)	ygiN	3719031..3719345 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
DXE54_RS18755 (DXE54_19335)	parE	3719393..3721285 (-)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=264980 DXE54_RS18730 WP_001221502.1 3715678..3716337(+) (ciaR) [Escherichia coli O145 strain RM10425-C1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=264980 DXE54_RS18730 WP_001221502.1 3715678..3716337(+) (ciaR) [Escherichia coli O145 strain RM10425-C1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365