Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DV179_RS10975 Genome accession   NZ_CP031331
Coordinates   1832989..1834215 (-) Length   408 a.a.
NCBI ID   WP_024465006.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M26263     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1827989..1839215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV179_RS10970 yccS 1830722..1832872 (-) 2151 WP_024465005.1 YccS family putative transporter -
  DV179_RS10975 pilU 1832989..1834215 (-) 1227 WP_024465006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DV179_RS10980 pilT 1834378..1835421 (-) 1044 WP_002218487.1 type IV pilus twitching motility protein PilT Machinery gene
  DV179_RS10985 - 1835541..1836227 (+) 687 WP_024465007.1 YggS family pyridoxal phosphate-dependent enzyme -
  DV179_RS10990 - 1836227..1836635 (+) 409 Protein_1794 RNA-binding protein -
  DV179_RS10995 proC 1836684..1837475 (+) 792 WP_024465008.1 pyrroline-5-carboxylate reductase -
  DV179_RS11000 dksA 1837622..1838038 (+) 417 WP_002231430.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45674.32 Da        Isoelectric Point: 7.1276

>NTDB_id=264565 DV179_RS10975 WP_024465006.1 1832989..1834215(-) (pilU) [Neisseria meningitidis strain M26263]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=264565 DV179_RS10975 WP_024465006.1 1832989..1834215(-) (pilU) [Neisseria meningitidis strain M26263]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACAGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(49-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

44.598

88.48

0.395
  pilU Pseudomonas stutzeri DSM 10701

44.663

87.255

0.39
  pilU Vibrio cholerae strain A1552

46.726

82.353

0.385

Multiple sequence alignment