Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   DW289_RS05960 Genome accession   NZ_CP031321
Coordinates   1191909..1192568 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1186909..1197568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DW289_RS05935 (DW289_05935) plsC 1187056..1187793 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  DW289_RS05940 (DW289_05940) parC 1188026..1190284 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  DW289_RS05950 (DW289_05950) ygiV 1190830..1191312 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  DW289_RS05955 (DW289_05955) ygiW 1191365..1191757 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  DW289_RS05960 (DW289_05960) ciaR 1191909..1192568 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  DW289_RS05965 (DW289_05965) qseC 1192565..1193912 (+) 1348 Protein_1100 quorum sensing histidine kinase QseC -
  DW289_RS05970 (DW289_05970) ygiZ 1193958..1194290 (-) 333 WP_000912120.1 DUF2645 family protein -
  DW289_RS05975 (DW289_05975) mdaB 1194609..1195193 (+) 585 WP_115431578.1 NAD(P)H-dependent oxidoreductase -
  DW289_RS05980 (DW289_05980) ygiN 1195224..1195538 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  DW289_RS05985 (DW289_05985) parE 1195586..1197478 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=264103 DW289_RS05960 WP_001221502.1 1191909..1192568(+) (ciaR) [Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=264103 DW289_RS05960 WP_001221502.1 1191909..1192568(+) (ciaR) [Escherichia coli strain ST2350 isolate Es_ST2350_SE1_NDM_03_2018]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365