Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CU052_RS08835 Genome accession   NZ_CP025537
Coordinates   1818242..1818706 (-) Length   154 a.a.
NCBI ID   WP_101904489.1    Uniprot ID   -
Organism   Vibrio harveyi strain 345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1813242..1823706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU052_RS08815 (CU052_09215) coaE 1813739..1814353 (-) 615 WP_101904488.1 dephospho-CoA kinase -
  CU052_RS08820 (CU052_09220) pilD 1814354..1815223 (-) 870 WP_045488712.1 A24 family peptidase Machinery gene
  CU052_RS08825 (CU052_09225) pilC 1815296..1816519 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  CU052_RS08830 (CU052_09230) pilB 1816553..1818238 (-) 1686 WP_005446257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CU052_RS08835 (CU052_09235) pilA 1818242..1818706 (-) 465 WP_101904489.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CU052_RS08845 (CU052_09245) nadC 1818971..1819858 (-) 888 WP_009700089.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CU052_RS08850 (CU052_09250) ampD 1819951..1820502 (+) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CU052_RS08855 (CU052_09255) pdhR 1820907..1821674 (+) 768 WP_009699648.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16054.29 Da        Isoelectric Point: 5.8347

>NTDB_id=262410 CU052_RS08835 WP_101904489.1 1818242..1818706(-) (pilA) [Vibrio harveyi strain 345]
MKTNKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAAAAMKLAVELCAHENASDAATFKTNCLSGS
NGVPSKIADLNDISIEAVAGSSGVNVIAKAIAVKGPIAANEQYTMASNYTTSGIEWTGSCQDSGGNAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=262410 CU052_RS08835 WP_101904489.1 1818242..1818706(-) (pilA) [Vibrio harveyi strain 345]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATTGTGGTTGCAATTATTGGTATTTT
GGCTGCATTTGCAGTACCTGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAGTTCCCAAAAGCAGCTGCAGCTA
TGAAGTTAGCAGTTGAATTGTGCGCGCACGAAAATGCTTCAGATGCAGCGACCTTTAAAACAAACTGCCTTTCTGGTAGC
AATGGAGTGCCTTCAAAGATTGCTGATTTAAACGATATATCTATTGAAGCTGTCGCTGGTTCCTCTGGCGTAAATGTAAT
TGCAAAAGCAATTGCAGTGAAAGGACCTATCGCAGCTAATGAACAATACACAATGGCATCAAACTACACTACAAGTGGTA
TTGAATGGACTGGCTCTTGTCAGGATTCTGGGGGGAACGCTCAGACTGATTACTGTCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.774

100

0.571
  pilA Haemophilus influenzae 86-028NP

41.259

92.857

0.383

Multiple sequence alignment